Endotoxin triggers the expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in the rat aorta in vivo

FEBS Letters
C SzabóH Ischiropoulos

Abstract

The free radicals nitric oxide (.NO) and superoxide (O2-) are known to react to form peroxynitrite (ONOO-), a highly reactive species. Peroxynitrite has been suggested to play an important role in the cellular damage associated with the overproduction of .NO, but there are very limited data regarding its in vivo formation. Here we demonstrate that injection of endotoxin into rats leads to the expression of an inducible isoform of .NO synthase (iNOS) in the thoracic aorta at 6 h and an increase in the circulating levels of nitrite/nitrate. Moreover, at the same time point, there is a marked increase in the immunoreactivity of nitrotyrosine, a marker of peroxynitrite in the aorta. The formation of nitrotyrosine was prevented by inhibiting the activity of NOS by NG-methyl-L-arginine in vivo. Our data suggest that during endotoxin shock, part of .NO, produced following the induction of iNOS, is converted into peroxynitrite in the vicinity of large blood vessels. The demonstration of the in vivo formation of peroxynitrite at sites of .NO overproduction may necessitate the development of novel and additional approaches for limiting or preventing .NO-related cytotoxic or vasodilatory actions during circulatory shock.

References

Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Jun 3, 1992·Journal of the National Cancer Institute·R G Kilbourn, O W Griffith
Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Dec 1, 1994·Journal of Leukocyte Biology·T M WizemannD L Laskin
Dec 1, 1994·The Journal of Clinical Investigation·I Y HaddadS Matalon
Jan 1, 1994·Methods in Enzymology·J S BeckmanJ P Crow
Aug 1, 1994·American Journal of Respiratory and Critical Care Medicine·H IschiropoulosS R Thom
May 1, 1994·Archives of Biochemistry and Biophysics·N W Kooy, J A Royall
Feb 1, 1994·Biological Chemistry Hoppe-Seyler·J S BeckmannC R White
May 1, 1993·Biochemical Society Transactions·J S Beckman, J P Crow

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Citations

Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J HellerD Lebrec
Apr 25, 2000·Archives of Biochemistry and Biophysics·G L SquadritoW A Pryor
Nov 18, 1998·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·I Mayers, D Johnson
Nov 9, 2006·Journal of Biomedical Science·Shyi-Shiaw JiauMao-Hsiung Yen
Jun 7, 2005·Experimental Neurology·Dominique LerouetIsabelle Margaill
May 16, 2000·Biochemical Pharmacology·A DaiberV Ullrich
Apr 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sadik SöğütOmer Akyol
Aug 30, 2001·European Journal of Pharmacology·S Cuzzocrea, R J Reiter
Dec 31, 2002·European Journal of Pharmacology·Annika M SvenssonLeif Jansson
Jul 31, 1998·European Journal of Pharmacology·C Szabó
Jul 20, 2002·Journal of Psychiatric Research·S Salih ZorogluOmer Akyol
Jan 1, 1997·Life Sciences·R B MuijsersF P Nijkamp
Dec 18, 2001·Pharmacology & Therapeutics·F FeihlL Liaudet
Jul 1, 1997·Journal of Neuroimmunology·R C van der VeenF M Hofman
Nov 24, 2001·Burns : Journal of the International Society for Burn Injuries·K SoejimaD L Traber
Feb 9, 2002·Burns : Journal of the International Society for Burn Injuries·Hiroyuki SakuraiDaniel L Traber
Oct 25, 1996·Journal of Chromatography. B, Biomedical Applications·Y KamisakiT Itoh
Sep 24, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·H LiuP T Kissinger
Mar 26, 1999·Journal of the American College of Cardiology·M FleschM Böhm
Jan 3, 2001·Free Radical Biology & Medicine·L A CallahanG S Supinski
Oct 26, 2002·Free Radical Biology & Medicine·Daniela Salvemini, Salvatore Cuzzocrea
Aug 6, 2002·Free Radical Biology & Medicine·Gediminas CepinskasPeter R Kvietys
Aug 2, 2007·Nature Reviews. Drug Discovery·Csaba SzabóRafael Radi
Feb 19, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G Sadeghi-HashjinF P Nijkamp
Mar 17, 2001·Clinical and Experimental Pharmacology & Physiology·R P LeeH I Chen
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D SalveminiT P Misko
Jun 8, 2001·Journal of Neurotrauma·J XuC Y Hsu

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