Endovascular Biopsy and Endothelial Cell Gene Expression Analysis of Dialysis Arteriovenous Fistulas: A Feasibility Study

Journal of Vascular and Interventional Radiology : JVIR
Hugh McGregorDaniel Cooke

Abstract

To demonstrate feasibility of endothelial cell (EC) biopsy from dialysis arteriovenous fistulas (AVFs) with the use of guidewires and to characterize gene expression differences between ECs from stenotic and nonstenotic outflow vein segments. Nine consecutive patients undergoing fistulography for AVF dysfunction from June to August 2016 were enrolled. ECs were biopsied with the use of guidewires from venous outflow stenoses and control outflow veins central to the stenoses. ECs were sorted with the use of flow cytometry, and the Fluidigm Biomark HD system was used for single-cell quantitative polymerase chain reaction (qPCR) analysis of gene expression. Forty-eight genes were assessed and were selected based on different cellular functions and previous literature. Linear mixed models (LMMs) were used to identify differential gene expression between the groups, and self-organizing maps (SOMs) were used to identify cell clusters based on gene coexpression profiles. A total of 219 and 213 ECs were sampled from venous outflow stenoses and control vein segments, respectively. There were no immediate biopsy-related complications. Forty-eight cells per patient were sorted for qPCR analysis. LMM identified 7 genes with different levels...Continue Reading

Citations

Apr 17, 2020·Frontiers in Cardiovascular Medicine·Nicholas W Chavkin, Karen K Hirschi
Dec 5, 2020·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Scott O TrerotolaTheodore F Saad
Dec 29, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Dillon GrantKewal Asosingh
Jul 24, 2021·Kidney International·Prabir Roy-ChaudhuryScott Trerotola

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