PMID: 12757878May 22, 2003Paper

Endoventricular porcine autologous myoblast transplantation can be successfully achieved with minor mechanical cell damage

Cardiovascular Research
Bénédicte ChazaudEmmanuel Teiger

Abstract

Transplantation of skeletal myogenic precursor cells (mpc) into the myocardium using a non-surgical procedure. Closed-chest mpc transplantation was assessed in pigs using the NOGA-Biosense device allowing both electromechanical mapping of the left ventricle (LV), and guided mpc injections through endocardium. We successively established that: (1) adequate preimplantation handling of mpc can be achieved when mpc are kept in 0.1% serum albumin-containing medium until implantation; (2) mpc are neither retained nor destroyed in the catheter or the needle and their passage does not affect their survival, growth and differentiation; (3) large numbers of autologous mpc can be actually transplanted in the LV myocardium by transendocardial route, as assessed by post-mortem examination of pigs injected with iron-loaded mpc; (4) cell injection into the myocardium does not induce conspicuous cell mortality since more than 80% of mpc recovered from LV tissue are alive 15 min after injection; (5) mpc injections can be guided into circumscribed LV targets such as infarcted areas, as assessed by comparison of map injection sites with location of iron-loaded mpc at post-mortem examination of LV myocardium. This new approach may pave the way for...Continue Reading

Citations

Mar 1, 2009·Journal of Cardiovascular Translational Research·Peter J Psaltis, Stephen G Worthley
Jun 19, 2010·Journal of Cardiovascular Translational Research·Peter J PsaltisStephen G Worthley
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Nov 19, 2010·Regenerative Medicine·Shazia DurraniKhawaja Husnain Haider
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May 25, 2006·Journal of Cell Science·Corinne SonnetBénédicte Chazaud

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