Endurance of methanogenic archaea in anaerobic bioreactors treating oleate-based wastewater

Applied Microbiology and Biotechnology
Andreia F SalvadorM Alcina Pereira

Abstract

Methanogenic archaea are reported as very sensitive to lipids and long chain fatty acids (LCFA). Therefore, in conventional anaerobic processes, methane recovery during LCFA-rich wastewater treatment is usually low. By applying a start-up strategy, based on a sequence of step feeding and reaction cycles, an oleate-rich wastewater was efficiently treated at an organic loading rate of 21 kg COD m(-3) day(-1) (50 % as oleate), showing a methane recovery of 72 %. In the present work, the archaeal community developed in that reactor is investigated using a 16S rRNA gene approach. This is the first time that methanogens present in a bioreactor converting efficiently high loads of LCFA to methane are monitored. Denaturing gradient gel electrophoresis profiling showed that major changes on the archaeal community took place during the bioreactor start-up, where phases of continuous feeding were alternated with batch phases. After the start-up, a stable archaeal community (similarity higher than 84 %) was observed and maintained throughout the continuous operation. This community exhibited high LCFA tolerance and high acetoclastic and hydrogenotrophic activity. Cloning and sequencing results showed that Methanobacterium- and Methanosaeta...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Jun 9, 2005·Research in Microbiology·Agnès GrabowskiChristian Jeanthon
Dec 8, 2005·Applied and Environmental Microbiology·Kevin E AshelfordAndrew J Weightman
Feb 1, 1987·Applied and Environmental Microbiology·I W Koster, A Cramer
Sep 8, 2006·Applied and Environmental Microbiology·Kevin E AshelfordAndrew J Weightman
Nov 17, 2007·Bioresource Technology·A J CavaleiroM Alves
May 30, 2009·Environmental Science & Technology·Ana J CavaleiroMadalena Alves
Jun 6, 2009·FEMS Microbiology Ecology·Diana Z SousaAlfons J M Stams
Jan 22, 2011·Archaea : an International Microbiological Journal·Sarah E HookBrian W McBride
Sep 1, 2009·Microbial Biotechnology·M Madalena AlvesAlfons J M Stams

❮ Previous
Next ❯

Citations

May 7, 2013·Applied and Environmental Microbiology·Diana Z SousaM Alcina Pereira
Jan 27, 2016·Environmental Science & Technology·Ana Júlia CavaleiroDiana Z Sousa
May 2, 2018·Applied Microbiology and Biotechnology·Antonio Castellano-HinojosaJesús González-López

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.