Abstract
Methanogenic archaea are reported as very sensitive to lipids and long chain fatty acids (LCFA). Therefore, in conventional anaerobic processes, methane recovery during LCFA-rich wastewater treatment is usually low. By applying a start-up strategy, based on a sequence of step feeding and reaction cycles, an oleate-rich wastewater was efficiently treated at an organic loading rate of 21 kg COD m(-3) day(-1) (50 % as oleate), showing a methane recovery of 72 %. In the present work, the archaeal community developed in that reactor is investigated using a 16S rRNA gene approach. This is the first time that methanogens present in a bioreactor converting efficiently high loads of LCFA to methane are monitored. Denaturing gradient gel electrophoresis profiling showed that major changes on the archaeal community took place during the bioreactor start-up, where phases of continuous feeding were alternated with batch phases. After the start-up, a stable archaeal community (similarity higher than 84 %) was observed and maintained throughout the continuous operation. This community exhibited high LCFA tolerance and high acetoclastic and hydrogenotrophic activity. Cloning and sequencing results showed that Methanobacterium- and Methanosaeta...Continue Reading
References
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1993·Electrophoresis·B G HäneH G Drexler
Oct 1, 1996·Journal of Bacteriology·U NübelH Backhaus
Mar 21, 1998·Applied and Environmental Microbiology·R GrosskopfW Liesack
Mar 22, 2001·Water Research·M M AlvesM Mota
Jul 27, 2001·Water Research·J A Lalman, D M Bagley
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Oct 2, 2004·Biotechnology and Bioengineering·M A PereiraM M Alves
Nov 4, 2004·The British Journal of Nutrition·Carla R SolivaAndrea Machmüller
Jun 9, 2005·Research in Microbiology·Agnès GrabowskiChristian Jeanthon
Sep 2, 2005·Biotechnology and Bioengineering·M A PereiraM M Alves
Dec 8, 2005·Applied and Environmental Microbiology·Kevin E AshelfordAndrew J Weightman
Mar 1, 1985·Applied and Environmental Microbiology·F RoyE Samain
Feb 1, 1987·Applied and Environmental Microbiology·I W Koster, A Cramer
Sep 8, 2006·Applied and Environmental Microbiology·Kevin E AshelfordAndrew J Weightman
Mar 22, 2007·FEMS Microbiology Ecology·Diana Z SousaM Madalena Alves
Nov 17, 2007·Bioresource Technology·A J CavaleiroM Alves
May 30, 2009·Environmental Science & Technology·Ana J CavaleiroMadalena Alves
Jun 6, 2009·FEMS Microbiology Ecology·Diana Z SousaAlfons J M Stams
Jun 1, 2004·FEMS Microbiology Ecology·Lorenzo BertinFabio Fava
Jan 22, 2011·Archaea : an International Microbiological Journal·Sarah E HookBrian W McBride
Sep 1, 2009·Microbial Biotechnology·M Madalena AlvesAlfons J M Stams
Citations
May 7, 2013·Applied and Environmental Microbiology·Diana Z SousaM Alcina Pereira
Jan 27, 2016·Environmental Science & Technology·Ana Júlia CavaleiroDiana Z Sousa
Jun 9, 2016·Microbial Biotechnology·Sérgio A SilvaDiana Z Sousa
Aug 4, 2016·Water Research·Ryan M ZielsBo H Svensson
Apr 16, 2015·FEMS Microbiology Ecology·Ryan M ZielsBo H Svensson
May 2, 2018·Applied Microbiology and Biotechnology·Antonio Castellano-HinojosaJesús González-López
Jun 19, 2020·Metabolites·Thi L HoVang V Le
Jan 5, 2021·Frontiers in Microbiology·Joana I AlvesAna J Cavaleiro