Enduring changes in tonic GABAA receptor signaling in dentate granule cells after controlled cortical impact brain injury in mice

Experimental Neurology
Jeffery A BoychukBret N Smith

Abstract

Changes in functional GABAAR signaling in hippocampus have previously been evaluated using pre-clinical animal models of either diffuse brain injury or extreme focal brain injury that precludes measurement of cells located ipsilateral to injury. As a result, there is little information about the status of functional GABAAR signaling in dentate granule cells (DGCs) located ipsilateral to focal brain injury, where significant cellular changes have been documented. We used whole-cell patch-clamp recordings from hippocampal slices to measure changes in GABAARs in dentate granule cells (DGCs) at 1-2, 3-5, and 8-13 weeks after controlled cortical impact (CCI) brain injury. Synaptic and tonic GABAAR currents (ITonicGABA) were measured in DGCs at baseline conditions and during application of the GABAAR agonist 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridine-3-ol hydrochloride (THIP) to assess in the function of δ subunit-containing GABAARs. DGCs ipsilateral to CCI exhibited no changes in the amplitude of resting ITonicGABA relative to DGCs after sham-injury or contralateral to CCI. In contrast, there was a significant reduction in the THIP-evoked ITonicGABA in DGCs ipsilateral to CCI at both time-points. Tonic GABAergic inhibition of DGCs i...Continue Reading

References

Oct 1, 1991·Journal of Neuroscience Methods·C E DixonR L Hayes
Feb 1, 1991·Trends in Pharmacological Sciences·H Lüddens, W Wisden
Feb 1, 1988·Journal of Neurophysiology·D S Weiss
Jan 1, 1988·Journal of Neurotrauma·J W Lighthall
Nov 1, 1984·The Journal of Physiology·G L CollingridgeB Robertson
Jan 1, 1994·Annual Review of Neuroscience·R L Macdonald, R W Olsen
Jul 15, 1996·The Journal of Physiology·G ChenA N van den Pol
Jul 22, 1999·Annals of the New York Academy of Sciences·P J WhitingK A Wafford
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·R M MihalekG E Homanics
Jan 5, 2002·Annals of Neurology·V SanthakumarI Soltesz
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 18, 2002·Epilepsia·Igor SpigelmanRichard W Olsen
Dec 7, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christoph RedeckerOtto W Witte
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brandon M StellIstvan Mody
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Valerie B CaraiscosBeverley A Orser
Apr 28, 2004·Trends in Neurosciences·Alexey SemyanovR Angus Silver
May 14, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claudio RiveraKai Kaila
Aug 28, 2004·Trends in Neurosciences·Istvan Mody, Robert A Pearce
Oct 1, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zechun PengCarolyn R Houser
Mar 2, 2005·Nature Reviews. Neuroscience·Mark Farrant, Zoltan Nusser

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Citations

May 14, 2017·Current Neurology and Neuroscience Reports·Rosalia PaternoAkiva S Cohen
Dec 2, 2017·Journal of Neuroscience Research·Eric J NeubergerVijayalakshmi Santhakumar
Jan 16, 2020·Epilepsy Currents·Devin K BinderUNKNOWN AES/NINDS Epilepsy Benchmarks Stewards
Nov 20, 2016·Epilepsy Currents·Bret N Smith
Sep 15, 2018·Molecular Psychiatry·Jennifer L McGuireRobert E McCullumsmith
Dec 27, 2016·Frontiers in Systems Neuroscience·Han-Chiao I ChenAkiva S Cohen
May 25, 2021·Experimental Neurology·Ryan A CloydBret N Smith

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