PMID: 8967358Apr 1, 1996Paper

Energetic basis for reduced contractile reserve in isolated rat hearts

The American Journal of Physiology
R Tian, J S Ingwall

Abstract

To study the relationship between myocardial energetics and contractile reserve, we acutely and selectively inhibited creatine kinase (CK) activity in isolated perfused rat hearts, using increasing doses of iodoacetamide. 31P nuclear magnetic resonance spectroscopy was used to measure intracellular pH and the concentrations of ATP, phosphocreatine, and inorganic phosphate. Contractile reserve was assessed as the increase of rate-pressure product (RPP) from baseline during high-calcium perfusion. Contractile reserve was reduced by 9, 35, and 72% in hearts with 26, 6, and 1% CK activity, respectively. An inverse linear relationship between RPP and the free energy release from ATP hydrolysis ([delta G approximately P[) was shown for all groups. Furthermore, the maximal RPPs of all hearts were achieved at the same level of [delta G approximately P[ (52-53 kJ/mol), which is equal to the free energy requirement of sarcoplasmic reticulum Ca2+ adenosine 5'-triphosphatase (ATPase). We suggest that inhibition of the CK reaction caused a decrease of [delta G approximately P[ which, in turn, limits the Ca(2+)-handling capacity of sarcoplasmic reticulum Ca2+ ATPase. In this way, the ability of the heart to increase its contractile performan...Continue Reading

Citations

Nov 13, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Marcia Silva QueirozFaramarz Ismail-Beigi
Mar 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Yong-Mei ChaMargaret M Redfield
Feb 4, 2005·American Journal of Physiology. Cell Physiology·Chad R HancockRonald A Meyer
Jun 19, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Julia FeyginJianyi Zhang
May 25, 2010·American Journal of Physiology. Heart and Circulatory Physiology·M Y MaslovR G Weiss
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J LiuJ Zhang
Aug 29, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Jason E PodrabskySteven C Hand
Dec 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Mohammad Nurulqadr JameelJianyi Zhang
Jun 25, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Mark A PeterzanOliver J Rider
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Darko PucarPetras P Dzeja
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Y YeR J Bache
May 11, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Lori A Gustafson, Johannes H G M Van Beek
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Apr 4, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Qingsong HuJianyi Zhang
Mar 11, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Julia FeyginJianyi Zhang
Jul 27, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Jagdip S JaswalGary D Lopaschuk
Jan 21, 2017·The Journal of Physiology·Michael KohlhaasChristoph Maack
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Michela Rossini, Riccardo Filadi
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May 3, 2002·Clinical and Experimental Pharmacology & Physiology·Jianyi Zhang
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Jun 19, 2019·FEBS Letters·Vasco SequeiraChristoph Maack

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