PMID: 3755058May 6, 1986Paper

Energetics of proline racemase: racemization of unlabeled proline in the unsaturated, saturated, and oversaturated regimes

Biochemistry
L M FisherJ R Knowles

Abstract

The interconversion of L- and D-proline catalyzed by proline racemase has been studied. The entire time course of the approach to equilibrium has been followed. After a short time the product concentration is significant, and the reaction runs under reversible conditions. As the total substrate concentration is increased, the system moves from the unsaturated regime into the saturated regime. At very high substrate levels under the reversible conditions used, the rate constant for substrate racemization falls, as the system moves into the "oversaturated" regime. Here, the net rate of the enzyme-catalyzed reaction is limited by the rate of return of the free enzyme from the form that liberates product back to the form that binds substrate. The results are analyzed in terms of the simple mechanism (table; see text) and illustrate the additional information that is available from reactions studied under reversible conditions. In the unsaturated region the value of the second-order rate constant kU (equivalent to kcat/Km) is 9 X 10(5) M-1 s-1 in each direction. In the saturated region, kcat = kcat = 2600 s-1 and Km = 2.9 mM. In the oversaturated region, the rate constant kO is 81 M s-1. The substrate concentration at which unsatura...Continue Reading

References

Dec 14, 1976·Biochemistry·W J Albery, J R Knowles
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Citations

Mar 25, 2009·Journal of the American Chemical Society·M Ashley SpiesJerome Baudry
Jun 6, 2009·Journal of the American Chemical Society·Amir Rubinstein, Dan Thomas Major
Mar 10, 2000·Biochemical and Biophysical Research Communications·C G Figueroa-Soto, E M Valenzuela-Soto
Sep 22, 2009·Archives of Biochemistry and Biophysics·Rosario A Muñoz-ClaresCarmina Montiel
Sep 1, 2008·Microbial Biotechnology·Stewart L Fisher
Jan 21, 1987·Journal of Theoretical Biology·W J Albery, J R Knowles
Jun 15, 1997·Archives of Biochemistry and Biophysics·D B Northrop, K L Rebholz
Jul 23, 2016·Applied Microbiology and Biotechnology·Christian FemmerSven Panke
Jun 11, 2014·The Journal of Physical Chemistry. B·Christophe BovignyMarco Stenta
Apr 20, 1993·Biochemistry·K A GalloJ R Knowles
Jun 15, 2007·Nature·Tomas LundqvistBoudewijn L M de Jonge
Jan 11, 2019·MedChemComm·Gary B EvansPeter C Tyler
Sep 12, 1998·The Journal of Biological Chemistry·Y K Cho, D B Northrop
Jul 14, 2009·Journal of Chemical Theory and Computation·Marco StentaAndrea Bottoni

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