PMID: 8612618Mar 1, 1996Paper

Energizing effects of illumination on the reactivities of lysine residues of the gamma subunit of chloroplast ATP synthase

European Journal of Biochemistry
M Komatsu-Takaki

Abstract

Incubation of chloroplast thylakoids with pyridoxal 5'-phosphate for a short time (5 s) modified the lysine residues of the gamma subunit of ATP synthase. Energization of thylakoids by illumination increased the reactivity of Lys24 by a factor of three and decreased the reactivity of Lys30 to 60%. The reactivities of these residues reached their maximum and minimum values, respectively, within 1 s after the onset of illumination. Illumination of thylakoids increased the reactivities of Lys222 and Lys231 in two steps by a factor of three. The first step was completed within 1 s and the second step was completed 20-30 s after the onset of illumination. In the presence of 10 mM NH4Cl, illumination of thylakoids did not change the reactivities of these lysine residues. These results suggest that the Lys24- and Lys30-containing region of the gamma subunit changes its conformation rapidly in response to delta mu H+ and that the Lys222- and Lys231-containing region of the gamma subunit changes its conformation in two steps in response to delta mu H+ formation.

References

Jan 1, 1977·Methods in Enzymology·P Bornstein, G Balian
Jun 1, 1985·The Biochemical Journal·G FalkJ E Walker
Aug 20, 1985·Journal of Molecular Biology·J E WalkerV L Tybulewicz
Jan 1, 1988·Physiological Reviews·A E Senior
Sep 6, 1984·Biochimica Et Biophysica Acta·J E WalkerN J Gay
Oct 25, 1984·Journal of Molecular Biology·V L TybulewiczJ E Walker
Jul 1, 1994·Trends in Biochemical Sciences·R A CapaldiS Wilkens

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Citations

Jan 1, 1996·Annual Review of Microbiology·G Deckers-Hebestreit, K Altendorf
Sep 27, 2002·Biochimica Et Biophysica Acta·Toru HisaboriDirk Bald
Jul 14, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Takatoshi SekiguchiToru Hisabori
May 2, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Felix BuchertPierre Joliot
Jun 6, 2000·Biochimica Et Biophysica Acta·M L RichterB Huchzermeyer

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