PMID: 2115863Aug 1, 1990Paper

Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells

Journal of Bacteriology
M B O'Hara, J H Hageman

Abstract

Bacterial cells degrade intracellular proteins at elevated rates during starvation and can selectively degrade proteins by energy-dependent processes. Sporulating bacteria can degrade protein with apparent first-order rate constants of over 0.20 h-1. We have shown, with an optimized [14C]leucine-labeling and chasing procedure, in a chemically defined sporulation medium, that intracellular protein degradation in sporulating cells of Bacillus subtilis 168 (trpC2) is apparently energy dependent. Sodium arsenate, sodium azide, carbonyl cyanide m-chlorophenylhydrozone, and N,N'-dicyclohexylcarbodiimide, at levels which did not induce appreciable lysis (less than or equal to 10%) over 10-h periods of sporulation, inhibited intracellular proteolysis by 13 to 93%. Exponentially growing cells acquired arsenate resistance. In contrast to earlier reports, we found that chloramphenicol (100 micrograms/ml) strongly inhibited proteolysis (68%) even when added 6 h into the sporulation process. Restricting the calcium ion concentration (less than 2 microM) in the medium had no effect on rates or extent of vegetative growth, strongly inhibited sporulation (98%), and inhibited rates of proteolysis by 60% or more. Inhibitors of energy metabolism,...Continue Reading

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Citations

Apr 1, 1991·Molecular Microbiology·V NorrisI B Holland
Aug 10, 2017·Critical Reviews in Biotechnology·Fabiano Jares ContesiniHélia Harumi Sato
Dec 6, 2018·Probiotics and Antimicrobial Proteins·Vladimir ElisashviliMichael L Chikindas
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christian von MeringPeer Bork
Jul 27, 1999·Journal of Bacteriology·D C DominguezJ H Hageman
Oct 15, 1996·Journal of Biochemical and Biophysical Methods·N G GallegosJ H Hageman
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·Y Kakinuma
Jul 1, 1996·Journal of Bacteriology·V NorrisR I Norman
Jun 1, 1992·Applied and Environmental Microbiology·S L Landuyt, E J Hsu
Nov 1, 1993·Journal of Bacteriology·K J SiranosianA D Grossman
Jun 1, 1997·Applied and Environmental Microbiology·J L PaceX Jiang

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