Energy conservation by Rhodothermus marinus respiratory complex I

Biochimica Et Biophysica Acta
Ana P BatistaManuela M Pereira

Abstract

A sodium ion efflux, together with a proton influx and an inside-positive DeltaPsi, was observed during NADH-respiration by Rhodothermus marinus membrane vesicles. Proton translocation was monitored by fluorescence spectroscopy and sodium ion transport by (23)Na-NMR spectroscopy. Specific inhibitors of complex I (rotenone) and of the dioxygen reductase (KCN) inhibited the proton and the sodium ion transport, but the KCN effect was totally reverted by the addition of menaquinone analogues, indicating that both transports were catalyzed by complex I. We concluded that the coupling ion of the system is the proton and that neither the catalytic reaction nor the establishment of the delta-pH are dependent on sodium, but the presence of sodium increases proton transport. Moreover, studies of NADH oxidation at different sodium concentrations and of proton and sodium transport activities allowed us to propose a model for the mechanism of complex I in which the presence of two different energy coupling sites is suggested.

References

Aug 1, 1978·Analytical Biochemistry·C Watters
Sep 5, 1997·Science·F R BlattnerY Shao
Jul 12, 2002·The Journal of Biological Chemistry·Anja C GemperliJulia Steuber
Jul 19, 2005·Biochimica Et Biophysica Acta·Ana M P MeloMiguel Teixeira
Aug 3, 2005·Extremophiles : Life Under Extreme Conditions·Snaedis H BjornsdottirJakob K Kristjansson
Feb 14, 2006·Science·Leonid A Sazanov, Philip Hinchliffe
Aug 15, 2006·Biochemical and Biophysical Research Communications·Ana M P MeloMiguel Teixeira
Jan 29, 2008·Protein and Peptide Letters·Meike StelterMargarida Archer

❮ Previous
Next ❯

Citations

Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jae Kyu LimVolker Müller
May 12, 2012·IUBMB Life·Ana P BatistaManuela M Pereira
Dec 22, 2010·Biochimica Et Biophysica Acta·Ana P Batista, Manuela M Pereira
Sep 26, 2013·Biochemical Society Transactions·Wojtek Steffen, Julia Steuber
May 14, 2011·Biochimica Et Biophysica Acta·Ulrich Brandt
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Mar 27, 2012·Biochimica Et Biophysica Acta·Ana P BatistaManuela M Pereira
Sep 25, 2012·Biochimica Et Biophysica Acta·Bruno C MarreirosManuela M Pereira
Dec 30, 2015·Biochimica Et Biophysica Acta·Paulo J CastroManuela M Pereira
Nov 11, 2017·Frontiers in Microbiology·Volker Müller, Verena Hess
Oct 21, 2019·Cellular and Molecular Life Sciences : CMLS·Marie Charlotte Schoelmerich, Volker Müller
Aug 3, 2012·The Journal of Biological Chemistry·Philippa G Roberts, Judy Hirst
Jan 6, 2021·Chemical Reviews·Filipa CalistoManuela M Pereira
Jul 6, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Oscar Gutiérrez-SanzAntonio L De Lacey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.