Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conductors.

Journal of Bacteriology
E Pavlasova, F M Harold

Abstract

Escherichia coli accumulates thiomethyl-beta-d-galactoside against a concentration gradient under anaerobic conditions. The accumulation was abolished by carbonylcyanide m-chlorophenylhydrazone, tetrachlorosalicylanilide, 2,4 dinitrophenol, and other uncouplers of oxidative phosphorylation even though oxidative phosphorylation would not be expected to occur anaerobically. In the presence of the uncouplers, the beta-galactoside carrier remained functional and catalyzed equilibration of thiomethylgalactoside across the membrane. The uncouplers did not inhibit the generation of adenosine triphosphate or protein turnover, or the accumulation of alpha-methylglucoside and glycerol by phosphorylation. We conclude that, at least anaerobically, uncouplers of oxidative phosphorylation do not interfere with energy metabolism in general, but prevent the utilization of metabolic energy for the active transport of galactosides. The uncouplers also facilitate passage of protons across the membrane. Various hypotheses are considered to explain why a proton-impermeable membrane may be required for active transport of galactosides and other substrates.

References

Apr 7, 1967·Biochemical and Biophysical Research Communications·S TanakaE C Lin
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R D SimoniS Roseman
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·G A ScarboroughE P Kennedy
Jul 1, 1967·Journal of Bacteriology·F M Harold, J R Baarda
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R A HarrisD E Green
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·U HopferT E Thompson
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C F Fox, E P Kennedy
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Sep 1, 1966·Journal of General Microbiology·R C Woodroffe, B E Wilkinson
Oct 26, 1967·Biochemical and Biophysical Research Communications·E Carafoli, C S Rossi
Jun 1, 1964·Bacteriological Reviews·E A DAWES, D W RIBBONS
Jun 1, 1965·Journal of Bacteriology·W W FORREST, D J WALKER
Mar 29, 1965·Biochimica Et Biophysica Acta·S HAYASHI, E C LIN
Mar 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·C F FoxE P Kennedy

❮ Previous
Next ❯

Citations

May 10, 1974·Annals of the New York Academy of Sciences·L Leive
Jan 1, 1972·Archiv für Mikrobiologie·H KaltwasserW R Conger
Feb 9, 1996·The Journal of Biological Chemistry·K AbeP C Maloney
Dec 1, 1970·The Journal of Membrane Biology·D S Feingold
Oct 17, 1977·European Journal of Biochemistry·R Serrano
Aug 1, 1972·Journal of Bioenergetics·I West, P Mitchell
Aug 17, 1973·European Journal of Biochemistry·H U Schairer, D Gruber
Jan 3, 1977·European Journal of Biochemistry·G ReberJ Deshusses
Dec 1, 1973·The Journal of Applied Bacteriology·H Galbraith, T B Miller
Jul 8, 2020·Antimicrobial Agents and Chemotherapy·M Ashley HudsonSteve W Lockless
Jun 15, 1974·European Journal of Biochemistry·K A DevorP Overath
Dec 1, 1971·The Journal of Membrane Biology·A Kepes
Jun 1, 1972·Bacteriological Reviews·F M Harold
Mar 1, 1976·Journal of Bacteriology·R SumradaT Cooper
Mar 1, 1976·Journal of Bacteriology·J L Flagg, T H Wilson
Sep 19, 1977·Biochimica Et Biophysica Acta·H T Jaspers, J van Steveninck
Nov 15, 1977·Biochimica Et Biophysica Acta·M Bentaboulet, A Kepes
Jun 28, 1982·Biochimica Et Biophysica Acta·D Kleiner
Oct 25, 1973·Biochimica Et Biophysica Acta·G van Thienen, P W Postma
Dec 1, 1977·Antimicrobial Agents and Chemotherapy·K S RosenthalD R Storm

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.