PMID: 7016267Feb 1, 1981Paper

Energy coupling mechanisms in host-grown Mycobacterium lepraemurium

Canadian Journal of Biochemistry
M IshaqueL Kato

Abstract

Energy coupling mechanisms of Mycobacterium lepraemurium isolated from Sprague-Dawley rats lepromata were investigated. Cell-free extracts catalyzed phosphorylation coupled to the oxidation of generated NADH, added NADH, and succinate yielding P/O ratios of approximately 0.8, 0.6, and 0.4, respectively. Ascorbate oxidation alone or in the presence of cytochrome c or N,N,N',N'-tetramethyl-p-phenylenediamine was not coupled to ATP synthesis. The oxidative phosphorylation was completely uncoupled by 2,4-dinitrophenol, 2,6-dibromophenol, pentachlorophenol, m-chlorocarbonylcyanide phenylhydrazone, dicumarol, and gramicidin at concentrations which did not cause any inhibition of oxygen uptake. While the NADH oxidation and associated phosphate esterification was markedly sensitive to rotenone and other flavoprotein inhibitors, these inhibitors had no effect, however, on the phosphorylation coupled to succinate oxidation. The respiratory chain inhibitors such as antimycin A or 2-n-heptyl-4-hydroxyquinoline-N-oxide, and cyanide were the potent inhibitors of the phosphorylation associated with the oxidation of NADH and succinate. The ATP formation coupled to the oxidation of NADH and succinate was also inhibited by oligomycin as well as ...Continue Reading

Citations

Nov 1, 1991·Research in Microbiology·M Ishaque
Nov 5, 2019·Veterinary Medicine and Science·Marc K KouamJunior O Moussala
May 16, 2019·PloS One·Daniela NumbergerHans-Peter Grossart
Jul 1, 2009·The New England Journal of Medicine·Shanta M Zimmer, Donald S Burke
Mar 29, 2011·Critical Reviews in Microbiology·Shengqiang Ge, Zhiliang Wang
Mar 13, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S L SwensonJ K McMillen
Jun 14, 2012·PLoS Pathogens·Pablo R MurciaJames L N Wood
Feb 6, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S BhattO G Pybus
Jan 1, 2013·Annual Review of Animal Biosciences·Makoto Ozawa, Yoshihiro Kawaoka
May 17, 2018·Emerging Microbes & Infections·Ping HeYing Chen

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