Energy-dependent stability of Shewanella oneidensis MR-1 biofilms.

Journal of Bacteriology
Renee M SavilleAlfred M Spormann

Abstract

Stability and resistance to dissolution are key features of microbial biofilms. How these macroscopic properties are determined by the physiological state of individual biofilm cells in their local physical-chemical and cellular environment is largely unknown. In order to obtain molecular and energetic insight into biofilm stability, we investigated whether maintenance of biofilm stability is an energy-dependent process and whether transcription and/or translation is required for biofilm dissolution. We found that in 12-hour-old Shewanella oneidensis MR-1 biofilms, a reduction in cellular ATP concentration, induced either by oxygen deprivation or by addition of the inhibitor of oxidative phosphorylation carbonyl cyanide m-chlorophenylhydrazone (CCCP), dinitrophenol (DNP), or CN(-), resulted in massive dissolution. In 60-hour-old biofilms, the extent of uncoupler-induced cell loss was strongly attenuated, indicating that the integrity of older biofilms is maintained by means other than those operating in younger biofilms. In experiments with 12-hour-old biofilms, the transcriptional and translational inhibitors rifampin, tetracycline, and erythromycin were found to be ineffective in preventing energy starvation-induced detachmen...Continue Reading

References

Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·U HopferT E Thompson
Jul 1, 1994·Applied and Environmental Microbiology·A Boyd, A M Chakrabarty
May 21, 1999·Science·J W CostertonE P Greenberg
Sep 3, 1999·Applied and Environmental Microbiology·C SternbergS Molin
Feb 13, 2001·Microbiology·I Sutherland
Aug 27, 2002·Emerging Infectious Diseases·Rodney M Donlan
Jun 7, 2003·Molecular Microbiology·Mikkel KlausenTim Tolker-Nielsen
May 4, 1962·Biochemical and Biophysical Research Communications·P G HEYTLER, W W PRICHARD
Nov 18, 2004·Journal of Bacteriology·Kai M ThormannAlfred M Spormann
Dec 3, 2004·Applied and Environmental Microbiology·Stephen M HuntPhilip S Stewart
Jan 11, 2005·Trends in Microbiology·Steven S BrandaRoberto Kolter
Jan 22, 2005·Journal of Bacteriology·Kai M ThormannAlfred M Spormann
May 17, 2005·Environmental Microbiology·Morten GjermansenTim Tolker-Nielsen
Mar 21, 2006·Journal of Bacteriology·Kai M ThormannAlfred M Spormann
May 9, 2006·Molecular Microbiology·Barbara I KazmierczakThomas S Murray
Aug 29, 2006·Applied and Environmental Microbiology·Tracy K TealDianne K Newman
Aug 19, 2007·FEMS Microbiology Letters·Jana MüllerAlfred M Spormann
Jan 29, 2008·Journal of Bacteriology·Vladimir JakovljevicLotte Søgaard-Andersen
Feb 21, 2008·Antimicrobial Agents and Chemotherapy·Rawya S Al-Dhaheri, L Julia Douglas
Mar 4, 2008·Microbiology·D PecharkiA Aa Scheie
May 20, 2008·Molecular Microbiology·Jason W Hickman, Caroline S Harwood
Jan 5, 1991·Biotechnology and Bioengineering·D H Applegate, J D Bryers
May 22, 2010·FEMS Microbiology Letters·Renée M SavilleAlfred M Spormann

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Citations

Sep 27, 2012·Applied Microbiology and Biotechnology·Bo Jiang, Yu Liu
Feb 22, 2012·Applied Biochemistry and Biotechnology·E SujaVayalam P Venugopalan
Oct 30, 1995·Molecular and Cellular Endocrinology·R BolandS Zanello
Aug 21, 2014·Applied Microbiology and Biotechnology·Anne SydowDirk Holtmann
May 20, 2015·Medical Molecular Morphology·Yuji MatsuzakiKouichi Sano
Aug 12, 2016·Nature Reviews. Microbiology·Hans-Curt FlemmingStaffan Kjelleberg
Apr 15, 2018·Applied and Environmental Microbiology·Kody L DuhlMichaela A TerAvest
Oct 1, 2014·Journal of Bacteriology·Tanya L JohnsonMaria Sandkvist
Jan 26, 2018·Scientific Reports·Cristina EscuderoRicardo Amils
Sep 29, 2017·NPJ Biofilms and Microbiomes·Robert NiederdorferHannes Peter
Jun 24, 2016·Microbiology Spectrum·Nicolas BarraudScott A Rice

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