PMID: 2104608Jan 1, 1990Paper

Energy-dependent uptake of 4-chlorobenzoate in the coryneform bacterium NTB-1

Journal of Bacteriology
P E GroenewegenJ A de Bont

Abstract

The uptake of 4-chlorobenzoate (4-CBA) in intact cells of the coryneform bacterium NTB-1 was investigated. Uptake and metabolism of 4-CBA were observed in cells grown in 4-CBA but not in glucose-grown cells. Under aerobic conditions, uptake of 4-CBA occurred with a high apparent affinity (apparent Kt, 1.7 microM) and a maximal velocity (Vmax) of 5.1 nmol min-1 mg of protein-1. At pH values below 7, the rate of 4-CBA uptake was greatly reduced by nigericin, an ionophore which dissipates the pH gradient across the membrane (delta pH). At higher pH values, inhibition was observed only with valinomycin, an ionophore which collapses the electrical potential across the membrane (delta psi). Under anaerobic conditions, no uptake of 4-CBA was observed unless an alternative electron acceptor was present. With nitrate as the terminal electron acceptor, 4-CBA was rapidly accumulated by the cells to a steady-state level, at which uptake of 4-CBA was balanced by excretion of 4-hydroxybenzoate. The mechanism of energy coupling to 4-CBA transport under anaerobic conditions was further examined by the imposition of an artificial delta psi, delta pH, or both. Uptake of 4-CBA was shown to be coupled to the proton motive force, suggesting a proto...Continue Reading

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Citations

Sep 1, 1992·FEMS Microbiology Reviews·M M Häggblom
Oct 1, 1990·The Journal of Applied Bacteriology·A H Neilson
Jan 1, 1990·Biodegradation·L C Commandeur, J R Parsons
Dec 9, 2003·Applied and Environmental Microbiology·A S YuroffW J Hickey
Aug 10, 2005·Canadian Journal of Microbiology·Emma R MasterWilliam W Mohn
Feb 13, 1999·Journal of Biotechnology·B Angelova, H P Schmauder
Jun 1, 1995·Microbiological Reviews·J SikkemaB Poolman
Feb 1, 1993·Journal of Bacteriology·H H LocherW N Konings
Jan 1, 1995·Applied and Environmental Microbiology·T Kuritz, C P Wolk
Mar 1, 1994·Applied and Environmental Microbiology·P W CoschiganoL Y Young
Dec 1, 1995·Applied and Environmental Microbiology·C B MiguezR A Macleod
Feb 1, 1992·Archives of Biochemistry and Biophysics·J L AllendeA Garrido-Pertierra

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