PMID: 9447924Feb 3, 1998Paper

Energy metabolism in cochlear outer hair cells in vitro

Hearing Research
B Puschner, J Schacht

Abstract

ATP levels in outer hair cells in vitro were measured using the luciferin/luciferase method. Hair cells were isolated from the guinea pig cochlea and maintained for 2 h in a balanced salt solution with 5.5 mM glucose. Ten to 20 cells sufficed for a robust and reproducible luminescence signal, indicating an ATP content of 6.2 +/- 0.4 fmol/cell. This ATP concentration is similar to that found in cultures of other cell types and agrees well with the classical measurements in freeze-dried preparations. The ATP levels were reduced by the following treatments: (1) the omission of glucose in the culture medium lowered ATP levels by 28%; (2) the inhibition of glycolysis by 2-deoxyglucose lowered ATP levels by 66%; (3) the inhibition of oxidative phosphorylation by carbonyl cyanide m-chlorophenylhydrazine (CCCP) lowered ATP levels by 75%, and (4) the inhibition of both pathways reduced the ATP content to non-detectable levels. Acetoacetate was able to restore ATP levels partially when glycolysis was inhibited. These results suggest that (1) the major pathway of ATP synthesis in outer hair cells is the aerobic metabolism of glucose; (2) endogenous energy stores (e.g. glycogen) can maintain ATP levels in the absence of glucose; and (3) ke...Continue Reading

References

May 1, 1977·The Laryngoscope·R ThalmannS Paloheimo
Nov 1, 1989·Brain and Language·D Kimura, N Watson
May 1, 1985·Hearing Research·H P ZennerU Schmitt
Jan 1, 1987·Hearing Research·G Zajic, J Schacht
Jan 11, 1985·Science·W E BrownellY de Ribaupierre
Feb 1, 1970·The Annals of Otology, Rhinology, and Laryngology·I ThalmannR Thalmann
Nov 1, 1970·The Laryngoscope·R ThalmannT H Comegys
Dec 1, 1972·The Laryngoscope·R ThalmannI Thalmann
Oct 1, 1973·The Laryngoscope·R ThalmannT Miyoshi
Jan 1, 1965·Cold Spring Harbor Symposia on Quantitative Biology·H Davis
Jan 8, 1996·FEBS Letters·C RichterC Franceschi
Oct 1, 1956·Archives of Biochemistry and Biophysics·A GREEN, W D MCELROY
May 4, 1962·Biochemical and Biophysical Research Communications·P G HEYTLER, W W PRICHARD

❮ Previous
Next ❯

Citations

Sep 2, 2003·Hearing Research·Birgit MazurekJohann Gross
Feb 23, 2012·The Journal of the Acoustical Society of America·Peter G JacobsEric A Wan
Apr 17, 2014·The Journal of Laryngology and Otology·S J DanielC V Rohlicek
Mar 30, 2016·Current Biology : CB·Kazuma MurakamiAlex C Keene
Jan 31, 2013·Hearing Research·Erik C Böttger, Jochen Schacht
Feb 1, 2015·Molecular Neurobiology·Stefan ReussNigel K Woolf
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Melanie Holmgren, Lavinia Sheets

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.