Energy of dissociation of lipid bilayer from the membrane skeleton of red blood cells

Biophysical Journal
W C Hwang, R E Waugh

Abstract

The association between the lipid bilayer and the membrane skeleton is important to cell function. In red blood cells, defects in this association can lead to various forms of hemolytic anemia. Although proteins involved in this association have been well characterized biochemically, the physical strength of this association is only beginning to be studied. Formation of a small cylindrical strand of membrane material (tether) from the membrane involves separation of the lipid bilayer from the membrane skeleton. By measuring the force required to form a tether, and knowing the contribution to the force due to the deformation of a lipid bilayer, it is possible to calculate the additional contribution to the work of tether formation due to the separation of membrane skeleton from the lipid bilayer. In the present study, we measured the tethering force during tether formation using a microcantilever (a thin, flexible glass fiber) as a force transducer. Numerical calculations of the red cell contour were performed to examine how the shape of the contour affects the calculation of tether radius, and subsequently separation work per unit area W(sk) and bending stiffness k(c). At high aspiration pressure and small external force, the r...Continue Reading

Citations

Sep 19, 2003·European Biophysics Journal : EBJ·D KuzmanB Zeks
Jul 6, 2005·European Biophysics Journal : EBJ·Veronika Kralj-IglicAles Iglic
May 15, 2009·Biomechanics and Modeling in Mechanobiology·Dirk Hartmann
Jul 9, 2010·Annals of Biomedical Engineering·Yong ChenJin-Yu Shao
Dec 29, 2000·Bioelectrochemistry·A IglicH Hägerstrand
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei HuangKyriacos A Athanasiou
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhangli PengSubra Suresh
Mar 4, 2000·Current Opinion in Hematology·D E Discher
Dec 17, 2009·PLoS Computational Biology·Matthew J PaszekDaniel A Hammer
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·F Brochard-WyartP Nassoy
Jul 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vadim A FrolovJoshua Zimmerberg
Aug 8, 2015·International Journal of Molecular Sciences·Amelia Ahmad Khalili, Mohd Ridzuan Ahmad
Feb 5, 2015·Biophysical Journal·Anagha DatarPramod A Pullarkat
Feb 12, 2008·Bioelectrochemistry·S Svetina, P Ziherl
Oct 29, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Grégory BeauneFrançoise Brochard-Wyart
Mar 18, 2009·Biophysical Journal·Erdem TabdanovJean-Paul Thiery
Aug 1, 2006·Biochemical and Biophysical Research Communications·Rehana Afrin, Atsushi Ikai
May 2, 2006·Biophysical Journal·Emily Glassinger, Robert M Raphael
Oct 29, 2003·Biophysical Journal·Hélène KarcherMohammad R Kaazempur-Mofrad
Nov 7, 2013·Biochimica Et Biophysica Acta·S MuñozG Alvarez
Feb 18, 2014·Advances in Colloid and Interface Science·S Svetina, B Zekš
Jun 5, 2014·Biophysical Journal·Richard J PowersAlexander A Spector
Aug 21, 2007·Biophysical Journal·Liselotte JauffredLene Broeng Oddershede
Apr 6, 2011·Biophysical Journal·Maria K PospieszalskaKlaus Ley
Feb 22, 2012·Biophysical Journal·Richard J PowersAlexander A Spector
May 6, 2008·Biophysical Journal·Julia SchmitzKay-Eberhard Gottschalk
Sep 27, 2005·Biophysical Journal·Mingzhai SunMichel Grandbois
Mar 1, 2003·Biophysical Journal·Jure DergancBostjan Zeks
May 23, 2002·Biophysical Journal·Robert M Hochmuth, Warren D Marcus
Aug 2, 2000·Biophysical Journal·S D RodgersD A Hammer
Feb 28, 2002·Biophysical Journal·Zhiwei LiWilliam E Brownell
May 29, 2007·Biophysical Journal·N Borghi, F Brochard-Wyart
Jan 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·P Ziherl, S Svetina
Apr 22, 2017·Physical Chemistry Chemical Physics : PCCP·Xiaohua ZhouShengli Zhang
Dec 10, 1999·Biophysical Journal·J Dai, M P Sheetz
May 4, 2007·Blood Cells, Molecules & Diseases·Ales IglicVeronika Kralj-Iglic
Sep 28, 2001·Nature·P C ZhangF Sachs
Oct 20, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Brenda FarrellWilliam E Brownell
Jan 5, 2010·Cellular and Molecular Bioengineering·Maria K Pospieszalska, Klaus Ley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.