PMID: 204195Mar 1, 1978

Energy relationships between cytosolic metabolism and mitochondrial respiration in rat heart

The American Journal of Physiology
K NishikiD F Wilson

Abstract

Isolated rat heart was perfused with Langendorff's retrograde perfusion method, while the oxygen consumption and the left ventricular pressure were monitored continually. The steady-state contents of metabolites in the cardiac tissue, freeze clamped under various work-load conditions, were determined and the concentrations of free cytosolic ADP and AMP were calculated from the near equilibrium in creatine phosphokinase and adenylate kinase reactions. Increasing respiratory rate with increasing load was accompanied by a fall in the cytosolic free [ATP]/[ADP][Pi] but little change in the mitochondrial free [NAD+]/[NADH]. The free energy of ATP hydrolysis was calculated from the concentrations of the adenine nucleotides and compared with the values computed from the measured turnover number for cytochrome c and redox state of the mitochondrial NAD couple according to a mathematical model. The agreement between the two values was good over a wide range of metabolic conditions, which provides further support for the proposed near-equilibrium model of mitochondrial respiration with control exerted at the cytochrome oxidase-oxygen reaction.

Citations

May 1, 1987·Biological Reviews of the Cambridge Philosophical Society·M D Brand, M P Murphy
Jan 1, 1982·The Journal of Membrane Biology·M Erecińska, D F Wilson
May 1, 1984·Acta Physiologica Scandinavica·R J Paul, P Hellstrand
Nov 1, 1980·Basic Research in Cardiology·J GiesenH Kammermeier
Feb 4, 2016·American Journal of Physiology. Endocrinology and Metabolism·David F Wilson
Oct 13, 2017·The Journal of Physiology·David F Wilson
Jan 1, 1991·European Journal of Applied Physiology and Occupational Physiology·C Y GuezennecP Portero
Dec 1, 1980·Journal of Neurochemistry·E Keller, J T Cummins
Jan 1, 1983·Annals of the New York Academy of Sciences·W E JacobusK M Vandegaer
Jun 1, 1987·Acta Physiologica Scandinavica·A KatzK Sahlin
Mar 4, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R SpringettD T Delpy
Jan 22, 2015·American Journal of Physiology. Endocrinology and Metabolism·David F Wilson
Jan 1, 1988·Annals of the New York Academy of Sciences·R O PoytonL E Farrell
Apr 1, 1994·Molecular and Cellular Biochemistry·Mary D Osbakken
Oct 6, 1986·FEBS Letters·A H FromK Uğurbil
Oct 30, 2016·Journal of Applied Physiology·David F Wilson
Sep 24, 2015·American Journal of Physiology. Endocrinology and Metabolism·David F Wilson
Jul 13, 2018·Journal of Applied Physiology·David F Wilson, Franz M Matschinsky
May 1, 1984·Basic Research in Cardiology·J R Williamson, K Kobayashi

Related Concepts

Adenine Nucleotides
Adenosine Triphosphate, Chromium Ammonium Salt
Diastolic Blood Pressure
Cytochromes
Cytoplasmic Matrix
Energy Metabolism
Heart
Hydrolysis
Mitochondria, Heart
Myocardium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.