PMID: 36970Jan 1, 1978

Energy transduction in the mitochondrionlike bacterium Paracoccus denitrificans during carbon- or sulphate-limited aerobic growth in continuous culture

Canadian Journal of Biochemistry
H G Lawford


Paracoccus denitrificans was grown in carbon-limited aerobic continuous culture (critical dilution rate (Dc) = 0.48 h-1). The molar growth yield for carbon (succinate or malate) was constant at about 60 over a broad dilution range (growth rate) from 0.10 to 0.48 h-1. Measurements of the stoichiometry of proton translocation associated with the oxidation of endogenous substrates yielded a ratio of protons ejected from the cell per atom of oxygen consumed(leads to H+:O) of 8.55 which decreased to 5.85 in the presence of piericidin A (PA), a specific inhibitor of NADH dehydrogenase (EC With starved cells, the observed leads to H+:O associated with the oxidation of added succinate in the presence of PA was 5.61. These observed leads to H:O's represent an underestimation since no correction was made for proton backflow during the short interval of respiratory activity. Aerobic growth of Pc. denitrificans in the chemostat becomes sulphate limited at entering concentrations of sulphate less than 300 is microM. Neither the maximum specific growth rate (measured at Dc) nor the observed molar growth yield for succinate decreased under sulphate limitation. The NADH oxidase in electron transport particles prepared from sulphate-...Continue Reading


Jun 1, 1988·Journal of Bioenergetics and Biomembranes·R A CapaldiW Yanamura
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B Ludwig, G Schatz
Jul 1, 1978·Archives of Microbiology·H W van Verseveld, A H Stouthamer
Apr 1, 1983·Antonie van Leeuwenhoek·D Castignetti, T C Hollocher

Related Concepts

Paracoccus denitrificans
Sulfates, Inorganic
Energy Transfer
Electron Transport
Carbonyl Cyanide m-Chlorophenyl Hydrazone

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