PMID: 589446Nov 4, 1977

Energy utilization in the uptake of catecholamines by synaptic vesicles and adrenal chromaffin granules

Brain Research
L TollB D Howard

Abstract

Several inhibitors of energy metabolism decreased the ATP-stimulated uptake of catecholamines by isolated synaptic vesicles from rat brain and by chromaffin granules from bovine adrenal medulla. Catecholamine uptake was inhibited by dinitrophenol, S-13 and oleic acid, which are known to block active transport by dissipating trans-membrane proton gradients. Thus a proton gradient appears to be involved in catecholamine transport. Both catecholamine uptake and vesicle-associated Ca2+/Mg2+-ATPase were inhibited by dicyclohexylcarbodiimide and tributyltin, which had previously been shown to inhibit the Ca2+/Mg2+-ATPase of mitochondria. However, mitochondrial ATPase was not involved in catecholamine uptake as oligomycin and aurovertin, more specific inhibitors of mitochondrial ATPase, did not affect catecholamine uptake. It is suggested that ATP stimulates catecholamine uptake by serving as a substrate for the ATPase. Activity of this enzyme causes translocation of protons across the vesicle membrane establishing a trans-membrane proton gradient. The proton gradient drives the transport of catecholamines.

References

Apr 1, 1975·The Biochemical Journal·C L BashfordG A Ritchie
Jan 1, 1975·Annual Review of Biochemistry·R D Simoni, P W Postma
Feb 14, 1966·Biochimica Et Biophysica Acta·N KIRSHNERD L Kamin
Jul 26, 1973·Naunyn-Schmiedeberg's Archives of Pharmacology·A Philippu, J Beyer
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·F A Henn, A Hamberger
Aug 16, 1974·Brain Research·A Faivre-BaumanP Benda
Jun 1, 1968·Biochemical Pharmacology·R W ColburnJ M Davis
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Apr 1, 1959·Biochimica Et Biophysica Acta·B B MARSH
Jul 1, 1962·The Journal of Biological Chemistry·N KIRSHNER

Citations

Jan 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·R HäuslerW Niedermaier
Nov 1, 1984·Biulleten' eksperimental'noĭ biologii i meditsiny·R N GlebovG N Kryzhanovskiĭ
Jun 13, 1979·Biochemical and Biophysical Research Communications·J E Rothlein, S M Parsons
Nov 30, 1981·Biochemical and Biophysical Research Communications·D C AndersonS M Parsons
May 19, 1978·Brain Research·D C Nelson-Krause, B D Howard
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S M Parsons, R Koenigsberger
Jan 1, 1985·Journal of Neurochemistry·S Naito, T Ueda
Dec 1, 1981·Journal of Neurochemistry·R G Sorensen, H R Mahler
Sep 1, 1986·British Journal of Pharmacology·A Vaccari
Jul 1, 1980·Journal of Neurochemistry·D M MichaelsonI Angel
Oct 3, 2006·Cell Metabolism·Patrick E MacDonaldPatrik Rorsman
Feb 12, 2013·Behavioural Brain Research·Zsuzsanna Callaerts-VeghRudi D'Hooge
Jul 21, 1981·Biochemistry·W P Melega, B D Howard
Jul 15, 1979·FEBS Letters·R M BucklandL M Wakefield
Oct 20, 1980·FEBS Letters·R G JohnsonA Scarpa
Aug 1, 1986·Journal of Neurochemistry·D Scherman

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Brain
Catecholamines
Bos indicus
Chromaffin Granule
Chromaffin System
Dinitrophenols
Energy Metabolism
Medihaler-Epi
Norepinephrine, (+, -)-Isomer

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