Engagement of the osteoclast integrin alpha v beta 3 by osteopontin stimulates phosphatidylinositol 3-hydroxyl kinase activity

Endocrinology
Keith A HruskaD Cheresh

Abstract

Osteoclast adhesion via integrin alpha v beta 3 to the bone matrix protein osteopontin (OPN) results in stimulation of bone resorption. We characterized an immediate signaling event that takes place upon osteoclast interaction with OPN. OPN binding to alpha v beta 3 results in the rapid production of the phosphoinositides (PtdInsP), including phosphatidylinositol triphosphate (PtdIns 3,4,5P3). Stimulation of 3,4,5-PtdInsP3 production by OPN was produced by increased activity of PtdInsP 3-OH kinase, which was found in immunoprecipitates formed by antibodies to integrin alpha v beta 3. The association of PtdIns 3-OH kinase with integrin may have been through association with c-src. The latter was present in immunoprecipitates formed by the antibodies to integrin alpha v beta 3, and src kinase was activated by OPN. These findings demonstrate a mechanism for rapid generation of cell signals upon matrix protein binding to alpha v beta 3, which resembles the mechanisms used by tyrosine kinase growth factor receptors.

Citations

Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena G Arias-SalgadoSanford J Shattil
Jan 19, 2010·The Journal of Biological Chemistry·Brooke K McMichaelBeth S Lee
Aug 1, 1997·The Journal of Clinical Investigation·A GuptaK A Hruska
May 9, 2001·The Journal of Clinical Investigation·X FengS L Teitelbaum
May 17, 2008·Journal of Materials Science. Materials in Medicine·Rupak M RajacharCecilia M Giachelli
Jan 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·T J Hall, T J Chambers
Dec 1, 2007·Cancer Metastasis Reviews·Philip Y Wai, Paul C Kuo
Apr 23, 2002·Journal of Cellular Biochemistry·S A KhanD T Denhardt
Oct 27, 2004·The Journal of Surgical Research·Philip Y Wai, Paul C Kuo
Aug 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan RammeltAchim Biewener
Sep 1, 2016·Molecular and Cellular Endocrinology·Jin-Man KimDaewon Jeong
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S W HuntY Shimizu
Apr 25, 2000·Biology of Reproduction·G A JohnsonF W Bazer
Aug 2, 2003·Biology of Reproduction·Greg A JohnsonThomas E Spencer
Nov 13, 1999·Nature Cell Biology·D P FelsenfeldM P Sheetz
Sep 25, 2004·Journal of Cellular Biochemistry·Cathy J AitkenGeoffrey C Nicholson
Aug 26, 1998·Stroke; a Journal of Cerebral Circulation·J A EllisonG Z Feuerstein
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuyuki HayashibaraToshiyuki Yoneda
Jun 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SudaN Takahashi
Jan 27, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A TetiK A Hruska
Apr 29, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M F PilkingtonS J Dixon
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M WozniakK A Hruska
Feb 12, 2015·Journal of Animal Science and Biotechnology·Greg A JohnsonFuller W Bazer
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H Nakamura, H Ozawa
Sep 4, 1999·Endothelium : Journal of Endothelial Cell Research·T L Thirkill, G C Douglas
Apr 16, 2003·The Journal of Cell Biology·Gotz von WichertMichael P Sheetz
May 4, 1999·The Journal of Cell Biology·T L BurgessD L Lacey

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