Engineered and wild-type L. lactis promote anti-inflammatory cytokine signalling in inflammatory bowel disease patient's mucosa

World Journal of Microbiology & Biotechnology
Saša SimčičRok Orel

Abstract

Dysbiosis of intestinal microbiota and aberrant inflammatory responses in gastrointestinal mucosa plays important roles in the development of inflammatory bowel disease (IBD). The purpose of this study was to demonstrate the probiotic activity of Lactococcus lactis and the ability of TNF-α-binding by recombinant L. lactis bearing TNF-α-binding affibodies. Various concentrations of recombinant L. lactis were exposed to TNF-α and its binding measured by ELISA. Mucosal biopsies of patients with active IBD were incubated with various L. lactis strains or E. coli DH5α strain and concentrations of TNF-α, IL-23, and IL-10 in the supernatants determined by ELISA. Recombinant L. lactis, at 1 × 109 and 1 × 108 CFU/mL, bound 22.6% and 18.4%, respectively of TNF-α (p < 0.05). When IBD-mucosa was incubated with any L. lactis strain at 1 × 109 CFU/mL, levels of TNF-α and IL-23 were significantly decreased and that of IL-10 increased relative to that for the sterile culture. Opposite trends were observed with E. coli cultures. Recombinant L. lactis at 1 × 108 CFU/mL bound as much as 62.8% (p = 0.026) of TNF-α in IBD-mucosa supernatants compared with the control strain. L. lactis strains are reported, for the first time, to induce an ex vivo a...Continue Reading

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Citations

Mar 7, 2020·Microbial Cell Factories·Zhao ZhouQipeng Yuan
Apr 7, 2021·Critical Reviews in Microbiology·Tejinder Pal Singh, Basavaprabhu Haranahalli Natraj
Jul 31, 2021·FEMS Microbiology Letters·Gargi Dey, Sohom Mookherjee

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Methods Mentioned

BETA
biopsies
ELISA
biopsy
flow
fluorescence microscopy

Software Mentioned

SPSS Statistics

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