Engineered Bacterial Bioreactor for Tumor Therapy via Fenton-Like Reaction with Localized H2 O2 Generation

Advanced Materials
Jin-Xuan FanXian-Zheng Zhang

Abstract

Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase localized H2 O2 generation. Following from this, magnetic Fe3 O4 nanoparticles are covalently linked to bacteria to act as a catalyst for a Fenton-like reaction, which converts H2 O2 to toxic hydroxyl radicals (•OH) for tumor therapy. In this constructed bioreactor, the Fenton-like reaction occurs with sustainably synthesized H2 O2 produced by engineered bacteria, and severe tumor apoptosis is induced via the produced toxic •OH. These results show that this bioreactor can achieve effective tumor colonization, and realize a self-supplied therapeutic Fenton-like reaction without additional H2 O2 provision.

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Citations

Oct 12, 2019·Chemical Communications : Chem Comm·Shuhua CaoXiaojun Peng
May 14, 2020·Advanced Healthcare Materials·Shuangqian YanBi-Feng Liu
Jul 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qiang SunMengjie Wu
Aug 19, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lile DongGuobin Hong
Sep 17, 2020·Advanced Healthcare Materials·Chen XuFu-Jian Xu
Oct 3, 2020·Advanced Materials·Di-Wei ZhengXian-Zheng Zhang
Sep 7, 2019·Advanced Materials·Qingling SongYun Zhang
Aug 28, 2019·Nanoscale·Ziliang DongLiangzhu Feng
Feb 6, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Weiqi WangHaijun Yu
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Nov 24, 2020·Chemical Society Reviews·Guo-Feng LuoXian-Zheng Zhang
Dec 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhengdi WangRuizheng Liang
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