Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio

Metabolic Engineering
Feiyan LiangPeter Lindblad

Abstract

The Calvin-Benson-Bassham (CBB) cycle is the main pathway to fix atmospheric CO2 and store energy in carbon bonds, forming the precursors of most primary and secondary metabolites necessary for life. Speeding up the CBB cycle theoretically has positive effects on the subsequent growth and/or the end metabolite(s) production. Four CBB cycle enzymes, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase), transketolase (TK) and aldolase (FBA) were selected to be co-overexpressed with the ethanol synthesis enzymes pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) in the cyanobacterium Synechocystis PCC 6803. An inducible promoter, PnrsB, was used to drive PDC and ADH expression. When PnrsB was induced and cells were cultivated at 65 µmol photons m-2 s-1, the RuBisCO-, FBP/SBPase-, TK-, and FBA-expressing strains produced 55%, 67%, 37% and 69% more ethanol and 7.7%, 15.1%, 8.8% and 10.1% more total biomass (the sum of dry cell weight and ethanol), respectively, compared to the strain only expressing the ethanol biosynthesis pathway. The ethanol to total biomass ratio was also increased in CBB cycle enzymes overexpressing strains. This study experimentally dem...Continue Reading

Citations

Jan 30, 2019·Life·Ann Magnuson
Jan 18, 2020·Plants·Valeria VecchiLuca Dall'Osto
Oct 3, 2020·International Journal of Molecular Sciences·Maciej CiebiadaMaurycy Daroch
Apr 29, 2019·Applied Microbiology and Biotechnology·Deviram GarlapatiArivalagan Pugazhendhi
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Mar 4, 2021·Microbial Cell Factories·Praveen C RamamurthyJoginder Singh
Mar 13, 2021·Physiologia Plantarum·Yagut AllahverdiyevaJorunn Skjermo

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