Engineered Escherichia coli strains as platforms for biological production of isoprene.

FEBS Open Bio
Hyeok-Won LeeHong-Weon Lee

Abstract

Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E. coli strains for improved isoprene production, including K-12 (DH5α, BW25113, W3110, MG1655, XL1-Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5-L fermentation, and scale-up fermentation (300 L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.

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Nov 16, 2017·Journal of Microbiology and Biotechnology·Hyeok-Won LeeHong-Weon Lee
Sep 25, 2019·Journal of Microbiology and Biotechnology·Hyeok-Won LeeHong-Weon Lee

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