Engineered hydrogels increase the post-transplantation survival of encapsulated hESC-derived midbrain dopaminergic neurons

Biomaterials
Maroof M AdilDavid V Schaffer

Abstract

Cell replacement therapies have broad biomedical potential; however, low cell survival and poor functional integration post-transplantation are major hurdles that hamper clinical benefit. For example, following striatal transplantation of midbrain dopaminergic (mDA) neurons for the treatment of Parkinson's disease (PD), only 1-5% of the neurons typically survive in preclinical models and in clinical trials. In general, resource-intensive generation and implantation of larger numbers of cells are used to compensate for the low post-transplantation cell-survival. Poor graft survival is often attributed to adverse biochemical, mechanical, and/or immunological stress that cells experience during and after implantation. To address these challenges, we developed a functionalized hyaluronic acid (HA)-based hydrogel for in vitro maturation and central nervous system (CNS) transplantation of human pluripotent stem cell (hPSC)-derived neural progenitors. Specifically, we functionalized the HA hydrogel with RGD and heparin (hep) via click-chemistry and tailored its stiffness to encourage neuronal maturation, survival, and long-term maintenance of the desired mDA phenotype. Importantly, ∼5 times more hydrogel-encapsulated mDA neurons survi...Continue Reading

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Citations

May 3, 2018·Advanced Healthcare Materials·Shupei QiaoWeiming Tian
Nov 18, 2018·British Journal of Pharmacology·K F BruggemanC L Parish
May 9, 2019·Artificial Organs·Alireza Dehqan NiriBasil Mujokoro
Sep 21, 2019·Tissue Engineering. Part a·Nicola L FrancisPrabhas V Moghe
Jul 4, 2020·Stem Cells Translational Medicine·Lila R Collins, Kelly A Shepard
Jul 22, 2018·Neural Regeneration Research·Niamh Moriarty, Eilís Dowd
Jan 9, 2019·Frontiers in Neuroanatomy·Natalia López-González Del ReyJavier Blesa
Jan 11, 2019·Stem Cells International·Taylor B Bertucci, Guohao Dai
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chao HeChangsheng Zhao
Sep 21, 2018·Frontiers in Neurology·Olivera RajkovicEmmanuel Pinteaux
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Akram ShafieeRassoul Dinarvand
Jan 31, 2021·Neurochemistry International·Sarah JarrinEilís Dowd
Feb 27, 2021·Trends in Pharmacological Sciences·Lidya Abune, Yong Wang
Mar 19, 2021·Neurochemistry International·Dimitri EigelBen Newland
Mar 23, 2021·Acta Biomaterialia·Sheng Yin, Yi Cao
Apr 20, 2021·APL Bioengineering·Mai T Ngo, Brendan A C Harley
May 13, 2021·ACS Applied Materials & Interfaces·Shreyas KuddannayaJeff W M Bulte
Jun 12, 2021·Frontiers in Bioengineering and Biotechnology·Amanda LiWeiwei He
Jul 2, 2021·Frontiers in Bioengineering and Biotechnology·Maria I Echeverria MolinaKyriakos Komvopoulos
Aug 1, 2021·Brain Research Bulletin·Wisberty J Gordián-VélezD Kacy Cullen
Aug 3, 2021·Progress in Neurobiology·Sebastiano Antonio RizzoBen Newland
Dec 14, 2019·Accounts of Chemical Research·Pei Liu, Evan W Miller
Oct 11, 2019·ACS Biomaterials Science & Engineering·Kayla J Wolf, Sanjay Kumar
Jan 19, 2021·ACS Biomaterials Science & Engineering·Hongjie ZongJingbo Yin
Oct 13, 2021·ACS Chemical Neuroscience·Samaneh MirzaeiJohn S Forsythe
Mar 28, 2020·Current Molecular Medicine·Maryam ShahiAli Abedelahi

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