Engineered protein coatings to improve the osseointegration of dental and orthopaedic implants

Biomaterials
Jordan RaphelSarah Heilshorn

Abstract

Here we present the design of an engineered, elastin-like protein (ELP) that is chemically modified to enable stable coatings on the surfaces of titanium-based dental and orthopaedic implants by novel photocrosslinking and solution processing steps. The ELP includes an extended RGD sequence to confer bio-signaling and an elastin-like sequence for mechanical stability. ELP thin films were fabricated on cp-Ti and Ti6Al4V surfaces using scalable spin and dip coating processes with photoactive covalent crosslinking through a carbene insertion mechanism. The coatings withstood procedures mimicking dental screw and hip replacement stem implantations, a key metric for clinical translation. They promoted rapid adhesion of MG63 osteoblast-like cells, with over 80% adhesion after 24 h, compared to 38% adhesion on uncoated Ti6Al4V. MG63 cells produced significantly more mineralization on ELP coatings compared to uncoated Ti6Al4V. Human bone marrow mesenchymal stem cells (hMSCs) had an earlier increase in alkaline phosphatase activity, indicating more rapid osteogenic differentiation and mineral deposition on adhesive ELP coatings. Rat tibia and femur in vivo studies demonstrated that cell-adhesive ELP-coated implants increased bone-implan...Continue Reading

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Oct 6, 1986·FEBS Letters·S Weiner, W Traub
Jan 1, 1995·Biosensors & Bioelectronics·H GaoH Sigrist
Nov 1, 1994·Journal of Cellular Biochemistry·S P BruderA I Caplan
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Jun 10, 1998·Journal of Biomedical Materials Research·S Szmukler-MonclerJ H Dubruille
Jul 29, 1998·Medical Engineering & Physics·J Y RhoP Zioupos
Jan 6, 1999·The Journal of Bone and Joint Surgery. American Volume·J U YooB Johnstone
Apr 2, 1999·Science·M F PittengerD R Marshak
Apr 29, 1999·The Journal of Arthroplasty·H KienapfelP Griss
Jun 15, 1999·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·C E MischM W Bidez
Dec 30, 1999·Biomaterials·D M FerrisR F Valentini
Nov 14, 2001·Reviews in Endocrine & Metabolic Disorders·J E Aubin
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John GoslineKen Savage
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D W UrryT Parker
Apr 9, 2002·Clinical Orthopaedics and Related Research·Robert W Bucholz
Oct 18, 2002·Advanced Drug Delivery Reviews·Zaki MegeedHamidreza Ghandehari
Apr 30, 2003·Arthritis Research & Therapy·Rocky S TuanRichard Tuli
May 14, 2003·Biomacromolecules·Kimberly Trabbic-CarlsonAshutosh Chilkoti
Jun 26, 2004·Journal of Dental Research·D BuserS G Steinemann
Oct 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jae I AhnKaren A Hasty
Dec 4, 2004·Clinical Orthopaedics and Related Research·John C ClohisyWilliam J Maloney
Dec 4, 2004·Clinical Orthopaedics and Related Research·Richard A BergerAaron G Rosenberg
Jan 11, 2005·Biomacromolecules·Sarah C HeilshornDavid A Tirrell
Apr 21, 2005·Journal of Dental Research·A J García, C D Reyes
Jul 5, 2005·The Journal of Bone and Joint Surgery. American Volume·Steven KurtzMichael Halpern
Sep 15, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Iqbal MassodiDrazen Raucher
Sep 21, 2005·Journal of Periodontology·Devorah Schwartz-AradEran Dolev
Sep 27, 2005·Micron : the International Research and Review Journal for Microscopy·Franchi MarcoOttani Vittoria

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Citations

Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·T T LiaoN Huang
Oct 28, 2018·Biotechnology Journal·Jean-Pierre AlcarazDonald K Martin
Apr 3, 2019·Advanced Healthcare Materials·Yao WangJin Kim Montclare
Jan 8, 2020·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jana StepanovskaHana Kolarova
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sławomir LachSylwia Rodziewicz-Motowidło
Dec 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Changcheng GaoBin Li
Nov 10, 2017·Tissue Engineering and Regenerative Medicine·Ozan KaramanUtku Kürşat Ercan
Nov 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xinkun ShenKaiyong Cai
Sep 29, 2020·Journal of Biomedical Materials Research. Part a·Tara B WigmostaMatt J Kipper
Nov 12, 2020·Military Medical Research·Shuai WeiXin-Long Ma
Feb 5, 2021·Biology·Nansi López-ValverdeJuan Manuel Ramírez
Feb 10, 2021·Materials·Yoshimitsu Okazaki, Shin-Ichi Katsuda
Dec 14, 2018·Acta Biomaterialia·Saba AtefyektaMartin Andersson
May 2, 2017·Current Opinion in Solid State & Materials Science·Matthew G Haugh, Sarah C Heilshorn
Apr 20, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kadie P NoblesRandall S Williamson
Apr 29, 2021·Biointerphases·Ahmet Engin PazarçevirenZafer Evis
Jun 3, 2021·Materials·Saturnino Marco LupiSilvana Rizzo
Jul 10, 2017·ACS Biomaterials Science & Engineering·Ana CivantosAnder Abarrategi
Oct 2, 2019·Journal of the American Chemical Society·Qi ChenRunhui Liu
May 8, 2017·ACS Biomaterials Science & Engineering·David Miranda-Nieves, Elliot L Chaikof
Jul 24, 2018·ACS Biomaterials Science & Engineering·J Kent Leach, Jacklyn Whitehead
Dec 16, 2020·ACS Biomaterials Science & Engineering·Logan D SouleXanthippi Chatzistavrou
Sep 24, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mingyue HanJiyao Li

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