Engineered protein switches for exogenous control of gene expression

Biochemical Society Transactions
Shaun Spisak, Marc Ostermeier

Abstract

There is an ongoing need in the synthetic biology community for novel ways to regulate gene expression. Protein switches, which sense biological inputs and respond with functional outputs, represent one way to meet this need. Despite the fact that there is already a large pool of transcription factors and signaling proteins available, the pool of existing switches lacks the substrate specificities and activities required for certain applications. Therefore, a large number of techniques have been applied to engineer switches with novel properties. Here we discuss some of these techniques by broadly organizing them into three approaches. We show how novel switches can be created through mutagenesis, domain swapping, or domain insertion. We then briefly discuss their use as biosensors and in complex genetic circuits.

Citations

Mar 23, 2002·Protein Science : a Publication of the Protein Society·Liskin Swint-KruseKathleen S Matthews
May 22, 2003·Journal of Molecular Biology·Oliver ScholzWolfgang Hillen
Mar 24, 2004·Nature Reviews. Microbiology·Douglas F Browning, Stephen J Busby
Nov 15, 2006·Cellular and Molecular Life Sciences : CMLS·Corey J WilsonKathleen S Matthews
Nov 28, 2006·Nature Chemical Biology·Edmund C SchwartzTom W Muir
Jul 25, 2007·Molecular Microbiology·Teca Calcagno GalvãoVíctor de Lorenzo
Mar 22, 2008·Journal of the American Chemical Society·Shuang-Yan TangPatrick C Cirino
Mar 10, 2009·Current Opinion in Microbiology·Liskin Swint-Kruse, Kathleen S Matthews
May 23, 2009·Nature Reviews. Molecular Cell Biology·Priscilla E M Purnick, Ron Weiss
Jun 23, 2009·Journal of Molecular Biology·Robert Daber, Mitchell Lewis
Apr 7, 2010·Annual Review of Biochemistry·Jeffrey A DietrichJay D Keasling
Jun 1, 2010·Protein Science : a Publication of the Protein Society·Leslie MilkMitchell Lewis
Jul 16, 2010·Molecular Systems Biology·Jian ZhanHaiyan Liu
Aug 2, 2011·Cell·Frank J PoelwijkSander J Tans
Jun 13, 2012·Chemistry : a European Journal·Jeung-Hoi Ha, Stewart N Loh
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Apr 17, 2013·Protein Engineering, Design & Selection : PEDS·Sarai MeyerCorey J Wilson
Jan 31, 2014·Chemistry : a European Journal·Jayoung MoonJeeyeon Lee
Mar 29, 2014·Biotechnology Journal·Nathaniel Roquet, Timothy K Lu
Jul 19, 2014·ACS Synthetic Biology·David L ShisMatthew R Bennett
Nov 8, 2014·Bioprocess and Biosystems Engineering·Irisappan GaneshSoon Ho Hong
Feb 3, 2015·Nature Biotechnology·Bernd ZetscheFeng Zhang
Apr 8, 2015·Nature Chemical Biology·Kevin M DavisDavid R Liu
May 20, 2015·Frontiers in Molecular Biosciences·Ashutosh PudasainiBrian D Zoltowski
Jun 16, 2015·Nature Biotechnology·Yuta NihongakiMoritoshi Sato
Jun 30, 2015·Nature Reviews. Microbiology·Peter J Rutledge, Gregory L Challis
Sep 1, 2015·Current Opinion in Biotechnology·James D WinklerRyan T Gill
Sep 22, 2015·Nature Methods·Shimyn Slomovic, James J Collins
Nov 2, 2015·Applied Microbiology and Biotechnology·Regina Mahr, Julia Frunzke
Dec 8, 2015·Nature Chemical Biology·Clement T Y ChanJames J Collins
Dec 23, 2015·Nature Methods·Noah D TaylorSrivatsan Raman
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jameson K Rogers, George M Church
Mar 12, 2016·Cell·Jens Nielsen, Jay D Keasling
May 3, 2016·Nature Biotechnology·Benjamin L OakesDavid F Savage
Nov 12, 2016·Journal of Industrial Microbiology & Biotechnology·Brecht De PaepeMarjan De Mey
Dec 13, 2016·Nature Chemical Biology·Kelly A SchwarzJoshua N Leonard
Feb 23, 2017·Nature Communications·Serif SenturkRaffaella Sordella

Related Concepts

biological signaling
Mutagenesis Process
Geographic Locations
Gene Circuits
Gene Expression
Gene Expression Regulation
Transcription Factor
Proteins

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Related Papers

Frontiers in Behavioral Neuroscience
Ahmed A Moustafa
Nature Chemical Biology
Yiyun Song
Trends in Microbiology
K Drlica, N R Perl-Rosenthal
© 2020 Meta ULC. All rights reserved