Engineered Resistance Against Papaya ringspot virus in Venezuelan Transgenic Papayas

Plant Disease
Gustavo FermínDennis Gonsalves

Abstract

Local varieties of papaya grown in the Andean foothills of Mérida, Venezuela, were transformed independently with the coat protein (CP) gene from two different geographical Papaya ringspot virus (PRSV) isolates, designated VE and LA, via Agrobacterium tumefaciens. The CP genes of both PRSV isolates show 92 and 96% nucleotide and amino acid sequence similarity, respectively. Four PRSV-resistant R0 plants were intercrossed or selfed, and the progenies were tested for resistance against the homologous isolates VE and LA, and the heterologous isolates HA (Hawaii) and TH (Thailand) in greenhouse conditions. Resistance was affected by sequence similarity between the transgenes and the challenge viruses: resistance values were higher for plants challenged with the homologous isolates (92 to 100% similarity) than with the Hawaiian (94% similarity) and, lastly, Thailand isolates (88 to 89% similarity). Our results show that PRSV CP gene effectively protects local varieties of papaya against homologous and heterologous isolates of PRSV.

References

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Citations

Aug 5, 2005·Annual Review of Phytopathology·Richard N Strange, Peter R Scott
Nov 22, 2007·Archives of Virology·T Fernández-RodríguezE Marys
Apr 24, 2014·TheScientificWorldJournal·Md Abul Kalam AzadNik Marzuki Sidik
Mar 21, 2021·Journal of Plant Physiology·Xiaobing ZhaoJingjing Yue

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