Engineering a Ca⁺⁺⁺-sensitive (bio)sensor from the pore-module of a potassium channel

Sensors
Mattia Lorenzo DiFrancescoAnna Moroni

Abstract

Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.

References

Jan 1, 1982·Annual Review of Pharmacology and Toxicology·B C Pressman, M Fahim
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·A MiyawakiR Y Tsien
Sep 15, 2001·Nature·H Bayley, P S Cremer
May 25, 2002·The Journal of Membrane Biology·S GazzarriniA Moroni
May 25, 2006·Chemistry & Biology·Amy E PalmerRoger Y Tsien
Jan 24, 2007·Biochemistry·Cinzia PagliucaDavid Parcej
Nov 21, 2007·Journal of Nanoscience and Nanotechnology·Erik Pierstorff, Dean Ho
Jan 6, 2009·Nature Methods·Jim BergGary Yellen
Sep 2, 2009·The Journal of General Physiology·Alessandra AbenavoliAnna Moroni
Nov 28, 2009·Nanotechnology·Qitao ZhaoXiyun Guan
Feb 27, 2013·The Journal of General Physiology·Cristina ArrigoniAnna Moroni

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Citations

Feb 25, 2018·The Journal of Membrane Biology·Alma E V AnderssonLucie Delemotte

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Methods Mentioned

BETA
biosensors
biosensor
PCR

Software Mentioned

- Voltage Clamp
TE
pCLAMP8

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