Sep 25, 2014

Engineering a circular riboregulator in Escherichia coli

BioRxiv : the Preprint Server for Biology
William RostainAlfonso Jaramillo

Abstract

Circular RNAs have recently been shown to be important gene expression regulators in mammalian cells. However, their role in prokaryotes remains elusive. Here, we engineered a synthetic riboregulator that self-splice to produce a circular molecule, exploiting group I permuted intron-exon (PIE) sequences. We demonstrated that the resulting circular riboregulator can activate gene expression, showing increased dynamic range compared to the linear form. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker. Thanks to the increased regulatory activity by higher stability, isolation due to self-splicing, and modularity of PIE, we envisage engineered circular riboregulators in further synthetic biology applications.

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Mentioned in this Paper

Biological Markers
Fluorescent Dyes
Exons
Isolation Aspects
Gene Expression
Fluorescent stain
Nuclear mRNA Cis Splicing, via Spliceosome
Circular Nucleus
RNA Splicing
Introns

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