Engineering a fungal peroxidase that degrades lignin at very acidic pH

Biotechnology for Biofuels
Elena Fernandez-FueyoAngel T Martínez

Abstract

Ligninolytic peroxidases are divided into three families: manganese peroxidases (MnPs), lignin peroxidases (LiPs), and versatile peroxidases (VPs). The latter two are able to degrade intact lignins, as shown using nonphenolic lignin model compounds, with VP oxidizing the widest range of recalcitrant substrates. One of the main limiting issues for the use of these two enzymes in lignocellulose biorefineries (for delignification and production of cellulose-based products or modification of industrial lignins to added-value products) is their progressive inactivation under acidic pH conditions, where they exhibit the highest oxidative activities. In the screening of peroxidases from basidiomycete genomes, one MnP from Ceriporiopsis subvermispora was found to have a remarkable acidic stability. The crystal structure of this enzyme recently became available and, after comparison with Pleurotus ostreatus VP and Phanerochaete chrysosporium LiP structures, it was used as a robust scaffold to engineer a stable VP by introducing an exposed catalytic tryptophan, with different protein environments. The variants obtained largely maintain the acidic stability and strong Mn(2+)-oxidizing activity of the parent enzyme, and the ability to oxid...Continue Reading

Citations

May 15, 2015·Applied Microbiology and Biotechnology·Elena Fernández-FueyoAngel T Martínez
Jul 14, 2016·Indian Journal of Microbiology·Radhika DeshmukhHemant J Purohit
May 4, 2016·Applied Microbiology and Biotechnology·Jacqueline MacDonaldEmma R Master
Apr 5, 2017·The Journal of Physical Chemistry. B·Sandra AcebesVictor Guallar
Jun 19, 2017·Biotechnology Advances·Angel T MartínezMiguel Alcalde
Oct 6, 2018·Chembiochem : a European Journal of Chemical Biology·Agathe BronikowskiVlada B Urlacher
Oct 27, 2018·Applied Microbiology and Biotechnology·Andrea CeciGeoffrey Michael Gadd
Sep 28, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bastien BissaroVincent G H Eijsink
Jul 22, 2019·Biotechnology Advances·Joseph C Stevens, Jian Shi
Oct 15, 2020·Enzyme and Microbial Technology·Odwa D V BikoWillem H van Zyl
Aug 28, 2021·Current Opinion in Biotechnology·Hye Jin Lim, Dong-Myung Kim

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

4BLK
1LGA

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