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Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage

Biotechnology and Bioengineering

May 9, 2012

Fang LiuWilfred Chen

Abstract

While protein purification has long been dominated by standard chromatography, the relatively high cost and complex scale-up have promoted the development of alternative non-chromatographic separation methods. Here we developed a new non-chromatographic affinity method for the purificat...read more

Mentioned in this Paper

Clostridium thermocellum
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Cohesins
Aggregation
Chromatography
Cytokinesis of the Fertilized Ovum
Protein Complex Location
CelA endoglucanase
1
45
Paper Details
References
  • References12
  • Citations12
12
  • References12
  • Citations12
12

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Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage

Biotechnology and Bioengineering

May 9, 2012

Fang LiuWilfred Chen

PMID: 22566125

DOI: 10.1002/bit.24545

Abstract

While protein purification has long been dominated by standard chromatography, the relatively high cost and complex scale-up have promoted the development of alternative non-chromatographic separation methods. Here we developed a new non-chromatographic affinity method for the purificat...read more

Mentioned in this Paper

Clostridium thermocellum
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Cohesins
Aggregation
Chromatography
Cytokinesis of the Fertilized Ovum
Protein Complex Location
CelA endoglucanase
1
45

Similar Papers Found In These Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Rotavirus Infections

Rotavirus infections often lead to gastroenteritis. Symptoms include diarrhea, vomiting, fever, and dehydration. Discover the latest research on rotavirus infections here.

Related Papers

Nature Protocols

Recombinant protein purification by self-cleaving aggregation tag

Nature ProtocolsApril 5, 2007
Wan-Yi WuDavid W Wood
Applied Microbiology and Biotechnology

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

Applied Microbiology and BiotechnologyDecember 6, 2012
Changhua ShiDavid W Wood
Paper Details
References
  • References12
  • Citations12
12
  • References12
  • Citations12
12
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