Hematopoietic stem cells reside in specific niches in the bone marrow and give rise to either more stem cells or maturing hematopoietic progeny depending on the signals provided in the bone marrow microenvironment. This microenvironment is comprised of cellular components as well as soluble constituents called cytokines. The use of cytokines alone for the ex vivo expansion of stem cells in flat, two-dimensional culture flasks, dishes or bags is inadequate and, given the three-dimensionality of the in vivo bone marrow microenvironment, inappropriate. Three-dimensional culture conditions can therefore provide an ex vivo mimicry of bone marrow, recapitulate the desired niche, and provide a suitable environment for stem cell expansion and differentiation. Choice of scaffold, manipulation and reproducibility of the scaffold properties and directed structuring of the niche, by choosing pore size and porosity may inform the resident stem cells of their fate in a directed fashion. The use of bioreactors for cultivation of hematopoietic cells will allow for culture control, optimization, standardization, scale-up, and a "hands-off" operation making the end-product dependable, predictable and free of contaminants, and therefore suitable ...Continue Reading
The microvasculature of the human bone marrow correlated with the distribution of hematopoietic cells. A computer-assisted three-dimensional reconstruction study
Culture perfusion schedules influence the metabolic activity and granulocyte-macrophage colony-stimulating factor production rates of human bone marrow stromal cells
Hematopoiesis on nylon mesh templates. Comparative long-term bone marrow culture and the influence of stromal support cells
Expansion of primitive human hematopoietic progenitors in a perfusion bioreactor system with IL-3, IL-6, and stem cell factor
Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin
Enhanced maintenance and retroviral transduction of primitive hematopoietic progenitor cells using a novel three-dimensional culture system
Clinical-scale human umbilical cord blood cell expansion in a novel automated perfusion culture system
A comparative study of the cell cycle status and primitive cell adhesion molecule profile of human CD34+ cells cultured in stroma-free versus porcine microvascular endothelial cell cultures
Extended culture of multipotent hematopoietic progenitors without cytokine augmentation in a novel three-dimensional device
Stirred culture of peripheral and cord blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications
Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity
Ex vivo expansion of human hematopoietic progenitors and cells to support high-dose chemoradiation therapy: five years of clinical experience
Cytokine-augmented culture of haematopoietic progenitor cells in a novel three-dimensional cell growth matrix
CD133+ cell selection is an alternative to CD34+ cell selection for ex vivo expansion of hematopoietic stem cells
Human cord cell hematopoiesis in three-dimensional nonwoven fibrous matrices: in vitro simulation of the marrow microenvironment
Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins.
Fabrication of porous biodegradable polymer scaffolds using a solvent merging/particulate leaching method
Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support
Preparation of macroporous biodegradable PLGA scaffolds for cell attachment with the use of mixed salts as porogen additives
3D culture of murine hematopoietic cells with spatial development of stromal cells in nonwoven fabrics
BMP4: its role in development of the hematopoietic system and potential as a hematopoietic growth factor
Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
Role of nutrient supply on cell growth in bioreactor design for tissue engineering of hematopoietic cells
Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation
Early exposure of murine embryonic stem cells to hematopoietic cytokines differentially directs definitive erythropoiesis and cardiomyogenesis in alginate hydrogel three-dimensional cultures
Microfabricated electrospun collagen membranes for 3-D cancer models and drug screening applications.
Compartmental hollow fiber capillary membrane-based bioreactor technology for in vitro studies on red blood cell lineage direction of hematopoietic stem cells.
In vitro reconstruction of a three-dimensional mouse hematopoietic microenvironment in the pore of polyurethane foam
Numerical fluid-dynamic optimization of microchannel-provided porous scaffolds for the co-culture of adherent and non-adherent cells
In silico regenerative medicine: how computational tools allow regulatory and financial challenges to be addressed in a volatile market.
From natural bone grafts to tissue engineering therapeutics: Brainstorming on pharmaceutical formulative requirements and challenges
In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry.
Biocompatible ion selective electrode for monitoring metabolic activity during the growth and cultivation of human cells
Three-dimensional culture of mouse bone marrow cells on stroma formed within a porous scaffold: influence of scaffold shape and cryopreservation of the stromal layer on expansion of haematopoietic progenitor cells
Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells
The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells
Ex vivo expansion of cord blood-CD34(+) cells using IGFBP2 and Angptl-5 impairs short-term lymphoid repopulation in vivo
Towards unravelling the kinetics of an acute myeloid leukaemia model system under oxidative and starvation stress: a comparison between two- and three-dimensional cultures
Organ-on-a-chip: development and clinical prospects toward toxicity assessment with an emphasis on bone marrow
Expansion of mouse hematopoietic progenitor cells in three-dimensional cocultures on frozen-thawed stromal cell layers formed within porous scaffolds
Three-dimensional ex vivo co-culture models of the leukaemic bone marrow niche for functional drug testing
Notch ligand presenting acellular 3D microenvironments for ex vivo human hematopoietic stem-cell culture made by layer-by-layer assembly
Combined multi-nozzle deposition and freeze casting process to superimpose two porous networks for hierarchical three-dimensional microenvironment
Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering
Fabrication of three-dimensional scaffolds using precision extrusion deposition with an assisted cooling device
Codelivery of Synovium-Derived Mesenchymal Stem Cells and TGF-β by a Hybrid Scaffold for Cartilage Regeneration
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.
Blood And Marrow Transplantation
The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.