Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

Journal of Biological Engineering
Josefine LiljeruhmAnthony C Forster

Abstract

Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong colors in ambient light. This endows CPs with certain advantages over FPs, such as instrument-free detection uncomplicated by ultra-violet light damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, CPs have found utility as genetic markers and in teaching, and are attractive for potential cell biosensor applications in the field. Most near-term applications of CPs require expression in a different domain of life: bacteria. However, it is unclear which of the eukaryotic CP genes might be suitable and how best to assay them. Here, taking advantage of codon optimization programs in 12 cases, we engineered 14 CP sequences (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple and gfasPurple) into a palette of Escherichia coli BioBrick plasmids. BioBricks comply with synthetic biology's most widely used, simplified, cloning standard. Differences in color intensities, maturation times and fitnes...Continue Reading

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Citations

May 22, 2019·PLoS Biology·Gaurav ByagathvalliM Saad Bhamla
Jun 16, 2019·Applied and Environmental Microbiology·Jacob E LazarusMatthew K Waldor
Jul 28, 2020·Microbial Biotechnology·Stamatios G DamalasVitor A P Martins Dos Santos
Oct 6, 2020·Frontiers in Microbiology·Ursula FelsPetra Van Damme
Aug 7, 2019·Protein and Peptide Letters·Jessica Tamayo-NuñezBernardo Franco
Jun 2, 2021·PloS One·Sanchita BhadraAndrew D Ellington
Jun 8, 2021·Biosensors & Bioelectronics·Michael MoraskieSylvia Daunert
Jan 7, 2022·AMB Express·Josué Daniel Mora-GarduñoBernardo Franco

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Methods Mentioned

BETA
FRET
biosensor
biosensors
PCR

Software Mentioned

Python
ImageJ
Adobe Photoshop
Java
GenScript

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