PMID: 7545410Aug 1, 1995Paper

Engineering a peptide epitope display system on filamentous bacteriophage

FEMS Microbiology Reviews
R N PerhamE Appella

Abstract

The genome of bacteriophage fd has been engineered to allow foreign amino acid sequences to be displayed in the exposed N-terminal segment of the major coat protein in the virus particle: small peptides can be encoded directly; larger peptides are encoded in hybrid virions, in which wild-type coat protein subunits are interspersed with coat proteins displaying the foreign peptides. Biophysical techniques, such as X-ray diffraction, indicate that the inclusion of the peptides can be achieved without significant disturbance to the helical parameters that define the protein-protein interactions in the assembled virion and the exposure of the peptides can be verified by analysing the susceptibility to attack by proteolytic enzymes. Peptide sequences from the V3 loop of the surface glycoprotein gp120 of HIV-1 strain MN (HIV-1MN) displayed in this way are remarkably effective structural mimics of the natural epitope. They are recognised by human HIV antisera and evoke high titres of virus-neutralizing antibodies in mice. Antibody production is stimulated by simultaneous inoculation with T cell epitopes similarly displayed on filamentous bacteriophage. The bacteriophage display system offers a powerful means of studying the immunologi...Continue Reading

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Citations

Sep 1, 1996·Protein Science : a Publication of the Protein Society·Z J RenL W Black
May 24, 2001·Journal of Molecular Biology·D M PedersonM R Slater
Aug 1, 1995·FEMS Microbiology Reviews·B H Lindqvist, S Naderi
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Feb 19, 2016·Advanced Healthcare Materials·Yanyan HuaiChuanbin Mao
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May 19, 2012·International Journal of Molecular Sciences·Sylvie DelhalleAndy Chevigné
Jan 23, 2003·Protein Engineering·V A PetrenkoT Quinn
Apr 25, 2008·Current Protocols in Immunology·David Enshell-Seijffers, Jonathan M Gershoni
Mar 30, 2007·FEMS Immunology and Medical Microbiology·Dina MascoloGiovanna Del Pozzo
May 1, 2021·Pharmaceuticals·Beata Zalewska-Piątek, Rafał Piątek
Apr 1, 1997·Chemical Reviews·George P. Smith, Valery A. Petrenko

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