Engineering acyl carrier protein to enhance production of shortened fatty acids

Biotechnology for Biofuels
Xueliang LiuJeffrey C Way

Abstract

The acyl carrier protein (ACP) is an essential and ubiquitous component of microbial synthesis of fatty acids, the natural precursor to biofuels. Natural fatty acids usually contain long chains of 16 or more carbon atoms. Shorter carbon chains, with increased fuel volatility, are desired for internal combustion engines. Engineering the length specificity of key proteins in fatty acid metabolism, such as ACP, may enable microbial synthesis of these shorter chain fatty acids. We constructed a homology model of the Synechococcus elongatus ACP, showing a hydrophobic pocket harboring the growing acyl chain. Amino acids within the pocket were mutated to increase steric hindrance to the acyl chain. Certain mutant ACPs, when over-expressed in Escherichia coli, increased the proportion of shorter chain lipids; I75 W and I75Y showed the strongest effects. Expression of I75 W and I75Y mutant ACPs also increased production of lauric acid in E. coli that expressed the C12-specific acyl-ACP thioesterase from Cuphea palustris. We engineered the specificity of the ACP, an essential protein of fatty acid metabolism, to alter the E. coli lipid pool and enhance production of medium-chain fatty acids as biofuel precursors. These results indicate t...Continue Reading

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Citations

Nov 9, 2016·Biotechnology for Biofuels·James C du Preez
Jan 28, 2017·Journal of Agricultural and Food Chemistry·Hyun Jeong LeeHan Min Woo
Mar 20, 2018·Applied and Environmental Microbiology·Marika ZiesackJeffrey C Way
Dec 9, 2017·Nature Biotechnology·Stephen SarriaPamela Peralta-Yahya
Jan 26, 2017·Frontiers in Microbiology·Aleksandr DemidenkoMarina G Kalyuzhnaya
Feb 15, 2019·International Journal of Molecular Sciences·Syed Ammar HussainYuanda Song
Apr 21, 2017·Current Opinion in Biotechnology·Amin ZargarJay D Keasling

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Software Mentioned

Agilent ChemStation

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