Engineering-Aligned 3D Neural Circuit in Microfluidic Device

Advanced Healthcare Materials
Seokyoung BangNoo Li Jeon

Abstract

The brain is one of the most important and complex organs in the human body. Although various neural network models have been proposed for in vitro 3D neuronal networks, it has been difficult to mimic functional and structural complexity of the in vitro neural circuit. Here, a microfluidic model of a simplified 3D neural circuit is reported. First, the microfluidic device is filled with Matrigel and continuous flow is delivered across the device during gelation. The fluidic flow aligns the extracellular matrix (ECM) components along the flow direction. Following the alignment of ECM fibers, neurites of primary rat cortical neurons are grown into the Matrigel at the average speed of 250 μm d(-1) and form axon bundles approximately 1500 μm in length at 6 days in vitro (DIV). Additionally, neural networks are developed from presynaptic to postsynaptic neurons at 14 DIV. The establishment of aligned 3D neural circuits is confirmed with the immunostaining of PSD-95 and synaptophysin and the observation of calcium signal transmission.

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Citations

Apr 30, 2016·Advanced Healthcare Materials·Masaya HagiwaraRina Nobata
Jun 15, 2016·Lab on a Chip·Mahdi KarimiMichael R Hamblin
Apr 22, 2017·Advanced Healthcare Materials·Midori Kato-NegishiShoji Takeuchi
Jan 20, 2018·Journal of Neural Engineering·Jordan ProxLiang Guo
Nov 14, 2018·Biofabrication·Seokyoung BangNoo Li Jeon
Sep 15, 2017·Advanced Healthcare Materials·Armin GerailiAmir Sanati-Nezhad
Sep 8, 2017·Advanced Healthcare Materials·Tatsuya OsakiRoger D Kamm
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Oct 17, 2019·Biomicrofluidics·Seokyoung BangHong Nam Kim
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Jun 23, 2021·Acta Biomaterialia·Seokyoung BangHong Nam Kim
Jul 20, 2021·Journal of Neural Engineering·Martina BrofigaPaolo Massobrio
Sep 5, 2020·Chemical Reviews·Adam M JorgensenAnthony Atala

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