Engineering and Characterization of Bacterial Nanocellulose Films as Low Cost and Flexible Sensor Material

ACS Applied Materials & Interfaces
Rahul MangayilSampo Tuukkanen

Abstract

Some bacterial strains such as Komagataeibacter xylinus are able to produce cellulose as an extracellular matrix. In comparison to wood-based cellulose, bacterial cellulose (BC) holds interesting properties such as biodegradability, high purity, water-holding capacity, and superior mechanical and structural properties. Aiming toward improvement in BC production titer and tailored alterations to the BC film, we engineered K. xylinus to overexpress partial and complete bacterial cellulose synthase operon that encodes activities for BC production. The changes in cell growth, end metabolite, and BC production titers from the engineered strains were compared with the wild-type K. xylinus. Although there were no significant differences between the growth of wild-type and engineered strains, the engineered K. xylinus strains demonstrated faster BC production, generating 2-4-fold higher production titer (the highest observed titer was obtained with K. xylinus-bcsABCD strain producing 4.3 ± 0.46 g/L BC in 4 days). The mechanical and structural characteristics of cellulose produced from the wild-type and engineered K. xylinus strains were analyzed with a stylus profilometer, in-house built tensile strength measurement system, a scanning ...Continue Reading

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Citations

Mar 22, 2018·Chemical Society Reviews·Wenshuai ChenZhuangjun Fan
Nov 7, 2017·Nanoscale·Minhyun JungSanghun Jeon
Jan 16, 2020·Critical Reviews in Biotechnology·Francisco German Blanco ParteKuan-Chen Cheng
Mar 19, 2019·Microbial Biotechnology·Paulina JacekStanisław Bielecki
Dec 28, 2019·Sensors·Carlo TrigonaAntonio Licciulli
Nov 14, 2019·Sensors·Nunzio CennamoAntonino Pollicino
Aug 23, 2019·Biotechnology and Bioengineering·Woo Dae JangSang Yup Lee
Nov 28, 2017·Bioprocess and Biosystems Engineering·Gizem BuldumAthanasios Mantalaris
Apr 16, 2021·Applied Microbiology and Biotechnology·Philipp Moritz FrickeTino Polen
Jun 3, 2021·Sensors·Kwan-Young JoungSung-Ho Cho
Dec 15, 2020·ACS Biomaterials Science & Engineering·Austin J GrahamBenjamin K Keitz
Nov 26, 2021·Bioengineered·Reshmy RAshok Pandey
Nov 5, 2020·Carbohydrate Polymers·Fernando G Torres, Gabriel E De-la-Torre

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