Engineering and systems-level analysis of Pseudomonas chlororaphis for production of phenazine-1-carboxamide using glycerol as the cost-effective carbon source

Biotechnology for Biofuels
Ruilian YaoXuehong Zhang

Abstract

Glycerol, an inevitable byproduct of biodiesel, has become an attractive feedstock for the production of value-added chemicals due to its availability and low price. Pseudomonas chlororaphis HT66 can use glycerol to synthesize phenazine-1-carboxamide (PCN), a phenazine derivative, which is strongly antagonistic to fungal phytopathogens. A systematic understanding of underlying mechanisms for the PCN overproduction will be important for the further improvement and industrialization. We constructed a PCN-overproducing strain (HT66LSP) through knocking out three negative regulatory genes, lon, parS, and prsA in HT66. The strain HT66LSP produced 4.10 g/L of PCN with a yield of 0.23 (g/g) from glycerol, which was of the highest titer and the yield obtained among PCN-producing strains. We studied gene expression, metabolomics, and dynamic 13C tracer in HT66 and HT66LSP. In response to the phenotype changes, the transcript levels of phz biosynthetic genes, which are responsible for PCN biosynthesis, were all upregulated in HT66LSP. Central carbon was rerouted to the shikimate pathway, which was shown by the modulation of specific genes involved in the lower glycolysis, the TCA cycle, and the shikimate pathway, as well as changes in ab...Continue Reading

References

Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K H SussK Adler
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Klaus M. Herrmann, Lisa M. Weaver
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·E Tjeerd van RijBen J J Lugtenberg
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Thomas F C Chin-A-WoengGuido V Bloemberg
Jan 9, 2007·Journal of Biotechnology·Katharina NöhWolfgang Wiechert
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 4, 2009·Nature Biotechnology·John E DueberJay D Keasling
Aug 7, 2009·Chembiochem : a European Journal of Chemical Biology·Matthias MentelWulf Blankenfeldt
Jun 28, 2011·Science·Camille J DelebecqueFaisal A Aldaye
Sep 13, 2011·Metabolic Engineering·Jamey D YoungJohn A Morgan
Aug 14, 2012·Environmental Microbiology·Dmitri V MavrodiLinda S Thomashow
Nov 28, 2012·Trends in Biotechnology·James M Clomburg, Ramon Gonzalez
Aug 24, 2013·Environmental Microbiology·Pablo I NikelVíctor de Lorenzo
Sep 13, 2014·Current Opinion in Structural Biology·Wulf Blankenfeldt, James F Parsons
Dec 3, 2014·Current Opinion in Biotechnology·Daniel C SévinUwe Sauer
Mar 4, 2015·Current Opinion in Biotechnology·Joerg M BuescherSarah-Maria Fendt
Sep 1, 2015·Current Opinion in Biotechnology·Allison G McAteeJamey D Young
Mar 24, 2016·Nature Chemistry·Ian WheeldonMatthew Sigman
Oct 28, 2016·Biotechnology and Bioengineering·Miguel SuásteguiZengyi Shao

❮ Previous
Next ❯

Citations

Nov 12, 2018·Microbial Cell Factories·Songwei WangXuehong Zhang
Jul 18, 2018·Applied Microbiology and Biotechnology·Muhammad BilalXuehong Zhang
Mar 19, 2019·Microbial Biotechnology·Ignacio Poblete-CastroPablo I Nikel
Sep 27, 2020·Biotechnology Journal·Tobias SchwanemannBenedikt Wynands
Nov 2, 2019·Current Opinion in Biotechnology·Toshihiro Obata
Apr 14, 2021·Journal of Agricultural and Food Chemistry·Wen-Hui LiuXue-Hong Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
genetic modifications
PCR

Software Mentioned

Excel

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.