Engineering Asymmetric Lipid Vesicles: Accurate and Convenient Control of the Outer Leaflet Lipid Composition

Langmuir : the ACS Journal of Surfaces and Colloids
Marie MarkonesSebastian Fiedler

Abstract

The asymmetric distribution of lipids between the two bilayer leaflets represents a typical feature of biological membranes. The loss of this asymmetry, for example the exposure of negatively charged lipids on the extracellular membrane leaflet of mammalian cells, is involved in apoptosis and occurs in tumor cells. Thus, the controlled production of asymmetric liposomes helps to better understand such crucial cellular processes. Here, we present an approach that allows us to design asymmetric model-membrane experiments on a rational basis and predict the fraction of exchanged lipid. In addition, we developed a label-free and nondestructive assay to quantify the asymmetric uptake of negatively charged lipids in terms of the zeta potential. This significantly enhances the applicability, impact, and predictive power of model membranes.

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Citations

Jun 6, 2020·The Journal of Biological Chemistry·Jim E HorneSheena E Radford
Aug 14, 2020·Soft Matter·Lionel Porcar, Yuri Gerelli
Sep 8, 2018·Nature Protocols·Milka DoktorovaDrew Marquardt
Jan 23, 2020·Biophysical Journal·Milka Doktorova
Dec 18, 2019·Biophysical Journal·Marie MarkonesHeiko Heerklotz
Feb 14, 2019·Biophysical Journal·Milka DoktorovaOlaf S Andersen
Jun 9, 2020·Biochimica Et Biophysica Acta. Biomembranes·R J ClarkeK Cao
Aug 2, 2020·Biophysical Journal·Lisa DietelHeiko Heerklotz
Oct 20, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Bingchen Li, Erwin London
Mar 1, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Ariane PeyretSébastien Lecommandoux
Aug 7, 2019·Accounts of Chemical Research·Erwin London
Apr 24, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Carolyn VargasSandro Keller
Dec 29, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Min-Kang HsiehJeffery B Klauda

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis