Engineering Bacillus thuringiensis bioinsecticides with an indigenous site-specific recombination system.

Applied and Environmental Microbiology
J A BaumC Gawron-Burke

Abstract

The cry genes of Bacillus thuringiensis encode a diverse group of crystal-forming proteins that exhibit insecticidal activity, particularly against the larvae of lepidopteran, coleopteran, and dipteran insects. The efficacy of B. thuringiensis-based biopesticides may be improved through the genetic manipulation of these genes. A gene transfer system has been developed for the introduction and maintenance of cloned insecticidal cry genes on small plasmids in B. thuringiensis. This vector system combines a B. thuringiensis plasmid replicon and an indigenous site-specific recombination system that allows for the selective removal of ancillary or foreign DNA from the recombinant bacterium after introduction of the Cry-encoding plasmid. The site-specific recombination system is useful for engineering strains with unique combinations of cry genes, resulting in new active ingredients with improved insecticidal properties.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 23, 1976·Molecular & General Genetics : MGG·G O HumphreysE S Anderson
Jan 1, 1992·Annual Review of Entomology·S S GillP V Pietrantonio
Dec 1, 1991·Gene·O Arantes, D Lereclus
Nov 1, 1991·Applied and Environmental Microbiology·M J RuparH de Barjac
Apr 1, 1990·Applied and Environmental Microbiology·A M Mettus, A Macaluso
Nov 1, 1990·Applied and Environmental Microbiology·J A BaumC Gawron-Burke
Jun 1, 1989·Microbiological Reviews·H Höfte, H R Whiteley
Apr 1, 1983·Journal of Bacteriology·J W KronstadH R Whiteley
Dec 1, 1993·Trends in Genetics : TIG·N J KilbyJ A Murray
Jan 1, 1995·Memórias do Instituto Oswaldo Cruz·S S Gill
Oct 1, 1995·Molecular Microbiology·J A Baum, T Malvar

❮ Previous
Next ❯

Citations

Jan 16, 2016·Applied Microbiology and Biotechnology·Yajun YuMeiying Gao
Jul 3, 2020·Toxins·Mikel Domínguez-ArrizabalagaPrimitivo Caballero
Aug 21, 2014·Protein Science : a Publication of the Protein Society·Artem G EvdokimovSonya Franklin
Dec 20, 2017·World Journal of Microbiology & Biotechnology·Salih KarabörklüZehra Busra Azizoglu
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.