Engineering bacterial motility towards hydrogen-peroxide

PloS One
Chelsea VirgileWilliam E Bentley

Abstract

Synthetic biologists construct innovative genetic/biological systems to treat environmental, energy, and health problems. Many systems employ rewired cells for non-native product synthesis, while a few have employed the rewired cells as 'smart' devices with programmable function. Building on the latter, we developed a genetic construct to control and direct bacterial motility towards hydrogen peroxide, one of the body's immune response signaling molecules. A motivation for this work is the creation of cells that can target and autonomously treat disease, the latter signaled by hydrogen peroxide release. Bacteria naturally move towards a variety of molecular cues (e.g., nutrients) in the process of chemotaxis. In this work, we engineered bacteria to recognize and move towards hydrogen peroxide, a non-native chemoattractant and potential toxin. Our system exploits oxyRS, the native oxidative stress regulon of E. coli. We first demonstrated H2O2-mediated upregulation motility regulator, CheZ. Using transwell assays, we showed a two-fold increase in net motility towards H2O2. Then, using a 2D cell tracking system, we quantified bacterial motility descriptors including velocity, % running (of tumble/run motions), and a dynamic net d...Continue Reading

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Citations

May 18, 2020·Nature Communications·Narendranath BhokishamWilliam E Bentley
Oct 22, 2020·IScience·Eric VanArsdaleWilliam E Bentley
Apr 19, 2020·Current Opinion in Biotechnology·Nikhil AggarwalMatthew Wook Chang
Apr 1, 2021·Advanced Materials·Jinyang LiGregory F Payne
Jul 2, 2021·Metabolic Engineering Communications·Kristina StephensWilliam E Bentley
Aug 12, 2021·Redox Report : Communications in Free Radical Research·Ché S Pillay, Nolyn John
Dec 2, 2021·Natural Product Reports·Jack A ConnollyRainer Breitling

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Methods Mentioned

BETA
Assay
PCR

Software Mentioned

Comsol
Prism
Matlab
ImageJ

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