Engineering Biological Approaches for Detection of Toxic Compounds: A New Microbial Biosensor Based on the Pseudomonas putida TtgR Repressor

Molecular Biotechnology
Manuel Espinosa-UrgelAna María Fernández-Escamilla

Abstract

Environmental contamination by toxic organic compounds and antimicrobials is one of the causes for the recent surge of multidrug-resistant pathogenic bacteria. Monitoring contamination is therefore the first step in containment of antimicrobial resistance and requires the development of simple, sensitive, and quantitative tools that detect a broad spectrum of toxic compounds. In this study, we have engineered a new microbial biosensor based on the ttgR-regulated promoter that controls expression of the TtgABC extrusion efflux pump of Pseudomonas putida, coupled to a gfp reporter. The system was introduced in P. putida DOT-T1E, a strain characterized by its ability to survive in the presence of high concentrations of diverse toxic organic compounds. This whole-cell biosensor is capable to detect a wide range of structurally diverse antibiotics, as well as compounds such as toluene or flavonoids.

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Citations

Jan 24, 2017·Metabolic Engineering·Dandan XiongShuang-Yan Tang
Oct 1, 2015·PloS One·Ana Maria Fernandez-EscamillaJuan Luis Ramos
Jan 8, 2021·Journal of Biological Engineering·Guey-Horng WangYing-Chien Chung
Jun 19, 2021·Chemical Society Reviews·Dong YiUwe T Bornscheuer

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