Engineering Cas9 for human genome editing.

Current Opinion in Structural Biology
Ian M Slaymaker, Nicole M Gaudelli

Abstract

Since the initial reports describing CRISPR-Cas9, labs across the globe have leveraged this valuable gene editing tool to alter the genomes of living cells. With the goal of generating more precise and efficient genome changes, scientists and engineers have mutated, evolved, and covalently altered Cas9 in order to predictably edit the genetic code. Here, we highlight recent advancements and contributions to the growing field of Cas9 engineering. We present key aspects of Cas9 engineering efforts focused on sgRNA manipulation, PAM-recognition, specificity, deaminase fusions, reverse-transcriptase fusions, and structural rearrangements of this important gene-modifying tool.

References

Mar 29, 2005·Journal of Molecular Evolution·Francisco J M MojicaElena Soria
Mar 24, 2007·Science·Rodolphe BarrangouPhilippe Horvath
Dec 20, 2008·Science·Luciano A Marraffini, Erik J Sontheimer
Nov 5, 2010·Nature·Josiane E GarneauSylvain Moineau
Apr 2, 2011·Nature·Elitza DeltchevaEmmanuelle Charpentier
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Jan 28, 2014·Nature Biotechnology·Yanfang FuJ Keith Joung
Jun 23, 2015·Nature·Benjamin P KleinstiverJ Keith Joung
Oct 24, 2015·Nature Reviews. Immunology·Gregory W Goldberg, Luciano A Marraffini
Nov 3, 2015·Nature Biotechnology·Benjamin P KleinstiverJ Keith Joung
Dec 3, 2015·Science·Ian M SlaymakerFeng Zhang
Feb 16, 2016·Cell·Hisato HiranoOsamu Nureki
Apr 19, 2016·Nature Reviews. Genetics·Shengdar Q Tsai, J Keith Joung
Mar 25, 2017·Nature Reviews. Drug Discovery·Hao YinDaniel G Anderson
Apr 5, 2017·Annual Review of Biophysics·Fuguo Jiang, Jennifer A Doudna
Sep 21, 2017·Nature·Janice S ChenJennifer A Doudna
Nov 29, 2017·Trends in Genetics : TIG·Payam A GammageMichal Minczuk
Feb 13, 2018·Nature Biotechnology·Antonio CasiniAnna Cereseto
Mar 20, 2018·Nature Biotechnology·Xiaosa LiJia Chen
May 17, 2018·Nature Communications·Mazhar Adli
Aug 8, 2018·Nature Communications·Jungjoon K LeeJin-Soo Kim
Aug 31, 2018·Nature Communications·Julie M Crudele, Jeffrey S Chamberlain
Sep 1, 2018·Science·Hiroshi NishimasuOsamu Nureki
Oct 9, 2018·Molecular Cell·Juanjuan YuanXing Chang
Oct 10, 2018·Nature Medicine·Lukas VilligerGerald Schwank
Mar 19, 2019·Annual Review of Biochemistry·Daesik KimJin-Soo Kim
Apr 25, 2019·Nature Communications·Shayesteh R FerdosiKaren S Anderson
May 11, 2019·Nature Communications·Kale KundertTanja Kortemme
May 16, 2019·Science Advances·Holly A ReesDavid R Liu

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