Engineering challenges in high density cell culture systems.

Cytotechnology
S S Ozturk

Abstract

High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The design of the cell retention is the key to performance of high density cell culture systems. Oxygenation and media design are also important for maximizing the cell number. In high density perfusion reactors, variable cell density, and hence the metabolic demand, require constant adjustment of perfusion rates. The use of cell specific perfusion rate (CSPR) control provides a constant environment to the cells resulting in consistent production. On-line measurement of cell density and metabolic activities can be used for the estimation of cell densities and the control of CSPR. Issues related to mass transfer and mixing become more important at high cell densities. Due to the difference in mass transfer coefficients for oxygen and CO(2), a significant accumulation of dissolved CO(2) is experienced with silicone tubing aeration. Also, mixing is observed to decrease at high densities. Base addition, if not properly done, c...Continue Reading

References

May 1, 1990·Bio/technology·M J Kearns
Sep 1, 1990·Biotechnology Progress·D W Murhammer, C F Goochee
Apr 1, 1986·Biochemical Society Transactions·T A SteitzJ M Grindley
Apr 1, 1994·Applied Microbiology and Biotechnology·J L MoreiraM J Carrondo
Nov 30, 1994·Annals of the New York Academy of Sciences·J L MoreiraM J Carrondo
Aug 5, 1988·Biotechnology and Bioengineering·M W GlackenA J Sinskey
Apr 25, 1991·Biotechnology and Bioengineering·S S Ozturk, B O Palsson
Feb 5, 1992·Biotechnology and Bioengineering·G M O'ConnorC L Cooney
Feb 20, 1992·Biotechnology and Bioengineering·M HülscherU Onken
Mar 25, 1993·Biotechnology and Bioengineering·S ZhangR E Spier
May 1, 1994·Biotechnology and Bioengineering·L Xie, D I Wang
Jun 20, 1994·Biotechnology and Bioengineering·W Zhou, W S Hu
Mar 20, 1995·Biotechnology and Bioengineering·P WuD Naveh
Jan 1, 1995·Cytotechnology·E C Beuvery

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Citations

Jul 9, 1999·Biotechnology and Bioengineering·P Jorjani, S S Ozturk
Jul 13, 2002·Biotechnology and Bioengineering·Jason J OsmanJulie Varley
Sep 6, 2001·Biotechnology and Bioengineering·J J OsmanJ Varley
Mar 12, 2008·Applied Microbiology and Biotechnology·Dheepak MaharajhSantosh Ramchuran
Nov 13, 2008·Cytotechnology·Alvin W Nienow
Dec 17, 2010·Cytotechnology·C SieblistA Lübbert
Oct 11, 2003·Biotechnology Advances·Sally McTaggart, Mohamed Al-Rubeai
Apr 1, 1997·Current Opinion in Biotechnology·W S Hu, J G Aunins
Oct 11, 2011·Biotechnology Advances·Jean-Christophe DrugmandSpiros N Agathos
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar
Dec 16, 2006·Biotechnology and Bioengineering·Chetan T GoudarKonstantin B Konstantinov
Oct 18, 2012·Biotechnology Progress·M Lourdes Velez-SuberbieDaniel G Bracewell
Apr 26, 2013·Biotechnology and Bioengineering·Cara Buchanan, Marissa Nichole Rylander
Sep 10, 2009·Biotechnology and Bioengineering·Timothy A BarrettGary J Lye
Jul 22, 2015·Journal of Biotechnology·Agata Villiger-OberbekJianguo Yang
Aug 19, 2015·Frontiers in Bioengineering and Biotechnology·Bastian Blombach, Ralf Takors
Dec 12, 2017·Biotechnology Progress·Retno Wahyu NurhayatiMasahiro Kino-Oka
May 22, 2018·Biotechnology Journal·Moritz K F WolfMassimo Morbidelli
Feb 22, 2018·Biotechnology Progress·Sen XuHao Chen
Jul 20, 2018·Biotechnology Journal·Mona BauschJochen B Sieck
Oct 24, 2018·Journal of Tissue Engineering and Regenerative Medicine·Jing LiuWeiping Ding
Jul 25, 2018·Biotechnology and Bioengineering·Moritz K F WolfMassimo Morbidelli
Jan 24, 2019·Bioprocess and Biosystems Engineering·Kahina MellahiOlivier Henry

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