PMID: 19929819Nov 26, 2009Paper

Engineering considerations for process development in mammalian cell cultivation

Current Pharmaceutical Biotechnology
Hu ZhangShengdi Fan

Abstract

Mammalian cell cultivation plays a great role in producing protein therapeutics in the last decades. Many engineering parameters are considered for optimization during process development in mammalian cell cultivation, only shear and mixing are especially highlighted in this paper. It is believed that shear stress due to agitation has been over-estimated to damage cells, but shear may result in nonlethal physiological responses. There is no cell damage in the regions where bubbles form, break up and coalescence, but shear stress becomes significant in the wake of rising bubbles and causes great damage to cells in bubble burst regions. Mixing is not sufficient to provide homogeneous dissolved oxygen tension, pH, CO2 and nutrients in large-scale bioreactors, which can bring severe problems for cell growth, product formation and process control. Scale-down reactors have been developed to address mixing and shear problems for parallel operations. Engineering characterization in conventional and recently developed scale-down bioreactors has been briefly introduced. Process challenges for cultivation of industrial cell lines in high cell densities as well as cultivation of stem cells and other human cells for regenerative medicine, t...Continue Reading

Citations

Aug 15, 2014·World Journal of Microbiology & Biotechnology·Fariba AkbariAhmad Yari Khosroushahi
Jan 19, 2012·Pharmacology & Therapeutics·Anna AstashkinaDavid W Grainger
Aug 24, 2010·Biotechnology Advances·Gabriel Potvin, Zisheng Zhang
Mar 8, 2012·Biotechnology Journal·Carl-Fredrik Mandenius, Mats Björkman
Jul 31, 2013·Biotechnology Progress·Arthur Nathan BrodskySarah W Harcum
Nov 6, 2012·Biotechnology and Bioengineering·David Bulnes-AbundisMario M Alvarez
Oct 7, 2016·Cytotechnology·Ya-Ching LinSheng-Chi Wu
Sep 13, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Russell P WolfeTabassum Ahsan
Dec 2, 2019·Bioprocess and Biosystems Engineering·Natalia V MenshutinaJoseph Boudrant
Jun 27, 2012·Molecular Biology Reports·Amir Ata SaeiAbolfazl Barzegari
Nov 2, 2021·Frontiers in Bioengineering and Biotechnology·Julian SchmitzAlexander Grünberger

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