Abstract
Triglycerides, traditionally sourced from plant oils, are heavily used in both industrial and healthcare applications. Commercially significant products produced from triglycerides include biodiesel, lubricants, moisturizers, and oils for cooking and dietary supplements. The need to rely upon plant-based production, however, raises concerns of increasing demand and sustainability. The reliance on crop yields and a strong demand for triglycerides provides motivation to engineer production from a robust microbial platform. In this study, Escherichia coli was engineered to synthesize and accumulate triglycerides. Triglycerides were produced from cell wall phospholipid precursors through engineered expression of two enzymes, phosphatidic acid phosphatase (PAP) and diacylglycerol acyltransferase (DGAT). A liquid chromatography-mass spectrometry (LC-MS) method was developed to analyze the production of triglycerides by the engineered E. coli strains. This proof-of-concept study demonstrated a yield of 1.1 mg/L triglycerides (2 g/L dry cell weight) in lysogeny broth medium containing 5 g/L glucose at 8 h following induction of PAP and DGAT expression. LC-MS results also demonstrated that the intracellular triglyceride composition of E...Continue Reading
References
Nov 1, 1988·Journal of Bacteriology·T Icho
Dec 1, 1994·Journal of Bacteriology·T A Voelker, H M Davies
Sep 1, 2000·Applied Microbiology and Biotechnology·H M AlvarezA Steinbüchel
Dec 28, 2002·The Journal of Biological Chemistry·Rainer Kalscheuer, Alexander Steinbüchel
Jul 21, 2004·Journal of Bacteriology·Jaiyanth DanielPappachan E Kolattukudy
Feb 8, 2006·Applied and Environmental Microbiology·Rainer KalscheuerAlexander Steinbüchel
Mar 29, 2008·Journal of Lipid Research·Yong-Mei Zhang, Charles O Rock
Sep 25, 2008·Metabolic Engineering·Xuefeng LuChaitan Khosla
Jan 27, 2009·Clinics in Dermatology·Publio Viola, Marzia Viola
Jan 30, 2010·Nature·Eric J SteenJay D Keasling
Oct 12, 2010·Science·Parayil Kumaran AjikumarGregory Stephanopoulos
Dec 15, 2010·The Journal of Biological Chemistry·Yi-Hsueh LuChristian R H Raetz
Jun 2, 2011·PloS One·Yangkai DuanXuefeng Lu
Jul 22, 2011·Analytical Chemistry·Susan S BirdBruce S Kristal
Aug 13, 2011·Nature·Clementina DellomonacoRamon Gonzalez
Nov 25, 2011·Enzyme and Microbial Technology·Eunyoung JeonJinwon Lee
Citations
Jan 7, 2014·Applied Microbiology and Biotechnology·Helge Jans Janßen, Alexander Steinbüchel
Jan 11, 2014·Biotechnology for Biofuels·Helge Jans Janßen, Alexander Steinbüchel
May 9, 2014·Current Microbiology·Min JiaLu-Ping Qin
Jan 17, 2015·Biotechnology for Biofuels·Santiago CombaHugo Gramajo
Dec 10, 2015·Metabolic Engineering·Jens PlassmeierAnthony J Sinskey
Jan 8, 2015·Frontiers in Bioengineering and Biotechnology·Ai-Qun YuMatthew Wook Chang
Sep 13, 2014·Applied Microbiology and Biotechnology·Martín A HernándezHéctor M Alvarez
Oct 16, 2015·Metabolic Engineering·Annika RöttigAlexander Steinbüchel
Apr 28, 2017·PloS One·Beatriz LázaroGabriel Moncalián
May 21, 2020·Plant & Cell Physiology·Motoki TanakaYukako Hihara