Engineering expression of bacterial polyphosphate kinase in tobacco for mercury remediation

Applied Microbiology and Biotechnology
Takeshi NagataHidemitsu Pan-Hou

Abstract

To develop the potential of plants to sequester and accumulate mercurials from the contaminated sites, we engineered a tobacco (Nicotiana tabacum) plant to express a bacterial ppk gene, encoding polyphosphate kinase (PPK), under control of a plant promoter. The designated plant expression plasmid pPKT116 that contains the entire coding region of ppk was used for Agrobacterium-mediated gene transfer into tobacco plants. A large number of independent transgenic tobacco plants were obtained, in some of which the ppk gene was stably integrated in the plant genome and substantially translated to the expected PPK protein in the transgenic tobacco. The presence of Hg2+ did not cause considerable morphological abnormalities in the transgenic tobacco, which grew, flowered, and set seed similarly to the wild-type tobacco on the medium containing normally toxic levels of Hg2+. The ppk-transgenic tobacco showed more resistance to Hg2+ and accumulated more mercury than its wild-type progenitors. These results suggest that ppk-specified polyphosphate has abilities to reduce mercury toxicity, probably via chelation mechanism, and also to accumulate mercury in the transgenic tobacco. Based on the results obtained in the present study, the expr...Continue Reading

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Citations

Apr 16, 2010·Applied Microbiology and Biotechnology·Takeshi NagataHidemitsu Pan-Hou
Apr 30, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Leah RosenfeldValeria C Culotta
Apr 7, 2009·Annual Review of Biochemistry·Narayana N RaoArthur Kornberg
Dec 5, 2006·Biological & Pharmaceutical Bulletin·Takeshi NagataHidemitsu Pan-Hou
Sep 2, 2009·Biological & Pharmaceutical Bulletin·Takeshi NagataHidemitsu Pan-Hou
Sep 9, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hidemitsu Pan-Hou
Oct 25, 2011·Journal of Hazardous Materials·Romeu FranciscoPaula Vasconcelos Morais
Mar 31, 2009·Current Opinion in Biotechnology·Oscar N Ruiz, Henry Daniell
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Ljudmilla BorisjukThomas Neuberger
May 15, 2012·Journal of Hazardous Materials·Jianxu WangLihai Shang
Apr 9, 2015·Environmental Science and Pollution Research International·Feng HeLiyuan Liang
Dec 28, 2010·International Journal of Food Microbiology·Yilei QianWalter D Calhoon
Jun 19, 2017·Biochemical and Biophysical Research Communications·Sheng XuRen Wang
Aug 2, 2008·The Journal of Toxicological Sciences·Takeshi NagataHidemitsu Pan-Hou
Dec 18, 2020·Letters in Applied Microbiology·C Shiny MatildaC Shanthi
Apr 4, 2021·International Journal of Environmental Research and Public Health·Emanuela D TiodarDorina Podar
May 29, 2010·Environmental Science & Technology·Julia M Diaz, Ellery D Ingall

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