Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of HEAT-SHOCK COGNATE 70

Plant Biotechnology Journal
Almudena Trapero-MozosMark A Taylor

Abstract

For many commercial potato cultivars, tuber yield is optimal at average daytime temperatures in the range of 14-22 °C. Further rises in ambient temperature can reduce or completely inhibit potato tuber production, with damaging consequences for both producer and consumer. The aim of this study was to use a genetic screen based on a model tuberization assay to identify quantitative trait loci (QTL) associated with enhanced tuber yield. A candidate gene encoding HSc70 was identified within one of the three QTL intervals associated with elevated yield in a Phureja-Tuberosum hybrid diploid potato population (06H1). A particular HSc70 allelic variant was linked to elevated yield in the 06H1 progeny. Expression of this allelic variant was much higher than other alleles, particularly on exposure to moderately elevated temperature. Transient expression of this allele in Nicotiana benthamiana resulted in significantly enhanced tolerance to elevated temperature. An TA repeat element was present in the promoter of this allele, but not in other HSc70 alleles identified in the population. Expression of the HSc70 allelic variant under its native promoter in the potato cultivar Desiree resulted in enhanced HSc70 expression at elevated tempera...Continue Reading

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Citations

Jun 6, 2018·Plant, Cell & Environment·Bernadetta Rina HastilestariSophia Sonnewald
Nov 15, 2018·Frontiers in Microbiology·Svetlana MakarovaMichael Taliansky
May 30, 2019·Frontiers in Plant Science·Keshav DahalBenoit Bizimungu
Jan 18, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lesley TorranceGlenn J Bryan
Jan 30, 2021·Frontiers in Plant Science·Günter G LehretzUwe Sonnewald

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Methods Mentioned

BETA
PCR
transgenic
nucleotide exchange
electrophoresis

Software Mentioned

MapQTL
Clustal Omega
GenStat
Sequencher
BLASTP
CLUSTAL O
MapQTL6
Phylogeny
fr
StepOne

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