Engineering interpenetrating network hydrogels as biomimetic cell niche with independently tunable biochemical and mechanical properties

Biomaterials
Xinming Tong, Fan Yang

Abstract

Hydrogels have been widely used as artificial cell niche to mimic extracellular matrix with tunable properties. However, changing biochemical cues in hydrogels developed-to-date would often induce simultaneous changes in mechanical properties, which do not support mechanistic studies on stem cell-niche interactions. Here we report the development of a PEG-based interpenetrating network (IPN), which is composed of two polymer networks that can independently and simultaneously crosslink to form hydrogels in a cell-friendly manner. The resulting IPN hydrogel allows independently tunable biochemical and mechanical properties, as well as stable and more homogeneous presentation of biochemical ligands in 3D than currently available methods. We demonstrate the potential of our IPN platform for elucidating stem cell-niche interactions by modulating osteogenic differentiation of human adipose-derived stem cells. The versatility of such IPN hydrogels is further demonstrated using three distinct and widely used polymers to form the mechanical network while keeping the biochemical network constant.

References

May 30, 1997·Science·C S ChenD E Ingber
May 29, 2000·Journal of Biomedical Materials Research·J ElisseeffR Langer
Dec 31, 2002·Biomaterials·Yong Doo ParkJeffrey A Hubbell
Aug 19, 2003·Biomaterials·Jeanie L Drury, David J Mooney
Dec 26, 2003·Biophysical Journal·Adam EnglerDennis Discher
May 18, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Charles R NuttelmanKristi S Anseth
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Jan 4, 2006·Cellular and Molecular Life Sciences : CMLS·A B MoshnikovaI P Beletsky
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Oct 18, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Shinji FujimotoAkinori Akaike
Oct 20, 2007·The EMBO Journal·Raheem PeeraniPeter W Zandstra
Dec 18, 2008·Pharmaceutical Research·Chien-Chi Lin, Kristi S Anseth
May 19, 2009·Acta Biomaterialia·Shalu Suri, Christine E Schmidt
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Feb 19, 2010·Angewandte Chemie·Charles E Hoyle, Christopher N Bowman
Sep 16, 2010·Chemistry of Materials : a Publication of the American Chemical Society·Cole A DeforestKristi S Anseth
Mar 12, 2011·Advanced Materials·Jason A Burdick, Glenn D Prestwich
Apr 20, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Laura HartmannCurtis W Frank
Dec 13, 2012·Nature Communications·Jason A Burdick, William L Murphy
Dec 28, 2009·Advanced Materials·Benjamin D FairbanksKristi S Anseth

❮ Previous
Next ❯

Citations

Jun 18, 2015·ACS Biomaterials Science & Engineering·Ryan M Schweller, Jennifer L West
Mar 15, 2016·Current Opinion in Biotechnology·Eliza MorrisCheemeng Tan
Jan 6, 2015·Journal of Applied Polymer Science·Chien-Chi LinHan Shih
Sep 23, 2014·Macromolecular Rapid Communications·Alexander DunduaAndreas Taden
Oct 9, 2014·Trends in Biotechnology·Annabel L ButcherMichelle L Oyen
May 23, 2015·Biochimica Et Biophysica Acta·Karin A JansenGijsje H Koenderink
Aug 19, 2015·Soft Matter·Richard A PlenderleithStephen Rimmer
Sep 23, 2014·Biomaterials·Hyun-Ji ParkSeung-Woo Cho
Mar 30, 2016·Progress in Polymer Science·Fa-Ming Chen, Xiaohua Liu
Nov 30, 2014·Applied Microbiology and Biotechnology·Ferdinand RuedingerThomas Scheper
Apr 3, 2016·Journal of Biomedical Materials Research. Part a·Béatrice LabatHassan Atmani
Feb 12, 2017·Journal of Biomedical Materials Research. Part a·Tingting XiaLi Yang
Sep 22, 2018·Biomaterials Science·Laura J MacdougallAndrew P Dove
May 22, 2019·Advanced Healthcare Materials·Michael D HuncklerAndrés J García
Mar 2, 2016·Biomedical Materials·Jun Hyuk BaeBong Geun Chung
Jun 16, 2016·Advanced Materials·Xinming Tong, Fan Yang
Aug 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shona O'BrienAndreas Heise
Jun 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kang-Ting HuangChun-Jen Huang
Apr 12, 2018·The International Journal of Artificial Organs·Roberta GentiliniPaola Petrini
Jan 12, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ugo MasulloMarta Madaghiele
Mar 26, 2021·The American Journal of Sports Medicine·Jin-A KimChul-Won Ha
Apr 13, 2021·Chemical Reviews·Xuanhe ZhaoGerman Parada
May 9, 2016·ACS Biomaterials Science & Engineering·Xinming TongFan Yang
Nov 30, 2021·Journal of Functional Biomaterials·Sahitya Chetan PandanaboinaAnindya Ghosh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.